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CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-03] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751203

CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-03] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD41 Recombinant Rabbit Monoclonal Antibody [PSH08-03] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CD41 aa 451-989.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 113 kDa

Positive Control

Human lung tissue lysate, Mouse spleen tissue lysate, Mouse lung tissue lysate, Mouse placenta tissue lysate, Rat spleen tissue lysate, Rat lung tissue lysate, human lung tissue, human spleen tissue, mouse placenta tissue, mouse spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

PSH08-03

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. ITGA2B, also known as CD41, encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. At least 38 disease-causing mutations in this gene have been discovered. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Background References

1. Dai L et al. Characterization of CD41(+) cells in the lymph node. Front Immunol. 2022 Aug

2. Sugimoto K et al. CD41(+) extracellular vesicles produced by avian thrombocytes contain microRNAs. Genes Cells. 2023 Dec

Subcellular Location

Membrane.

UNIPROT

SwissProt: P08514 Human

SwissProt: Q9QUM0 Mouse

Entrez Gene: 685269 Rat

Synonyms

antigen CD41 antibody

BDPLT16 antibody

BDPLT2 antibody

CD41 antibody

CD41B antibody

form 2 antibody

GP2B antibody

GPalpha IIb antibody

GPIIb antibody

GT antibody

Expand

Images

  • This data was developed using <a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of CD41 on different lysates with Rabbit anti-CD41 antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human lung tissue lysate<br />Lane 2: Mouse spleen tissue lysate<br />Lane 3: Mouse lung tissue lysate<br />Lane 4: Mouse placenta tissue lysate<br />Lane 5: Rat spleen tissue lysate<br />Lane 6: Rat lung tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 113 kDa<br />Observed band size: 113-130 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA722940, the same antibody clone in a different buffer formulation.
    Western blot analysis of CD41 on different lysates with Rabbit anti-CD41 antibody (HA722940) at 1/2,000 dilution.

    Lane 1: Human lung tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Mouse lung tissue lysate
    Lane 4: Mouse placenta tissue lysate
    Lane 5: Rat spleen tissue lysate
    Lane 6: Rat lung tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 113 kDa
    Observed band size: 113-130 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722940) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CD41 antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722940, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CD41 antibody (HA722940) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722940) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD41 antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722940, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD41 antibody (HA722940) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722940) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-CD41 antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722940, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-CD41 antibody (HA722940) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722940) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD41 antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722940, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD41 antibody (HA722940) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722940) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD41 antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722940" style="font-weight: bold;text-decoration: underline;">HA722940</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA722940, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD41 antibody (HA722940) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722940) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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