Engrailed 1 Recombinant Rabbit Monoclonal Antibody [PSH08-17] - BSA and Azide free
Catalog# HA751208
Engrailed 1 Recombinant Rabbit Monoclonal Antibody [PSH08-17] - BSA and Azide free
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Engrailed 1 Recombinant Rabbit Monoclonal Antibody [PSH08-17] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Engrailed 1 aa 1-392.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, SH-SY5Y.
Conjugation
unconjugated
Clone Number
PSH08-17
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Homeobox protein engrailed-1 is a protein that in humans is encoded by the EN1 gene. Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the engrailed (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system and the limbs.
Background References
1. Mascharak S et al. Preventing Engrailed-1 activation in fibroblasts yields wound regeneration without scarring. Science. 2021 Apr
2. Xu J et al. Engrailed-1 Promotes Pancreatic Cancer Metastasis. Adv Sci (Weinh). 2024 Feb
Subcellular Location
Nucleus.
Synonyms
En 1 antibody
EN1 antibody
Engrailed 1 antibody
Engrailed antibody
Engrailed homeobox 1 antibody
Engrailed homolog 1 antibody
HME1_HUMAN antibody
Homeobox protein en-1 antibody
Homeobox protein engrailed 1 antibody
Homeobox protein engrailed-1 antibody
ExpandImages
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This data was developed using HA722960, the same antibody clone in a different buffer formulation.
Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA722960) at 1/2,000 dilution.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: C6 cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722960) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722960, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of SH-SY5Y cells labeling Engrailed 1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722960, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"