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NK1.1 Recombinant Rabbit Monoclonal Antibody [PSH08-47] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751222

NK1.1 Recombinant Rabbit Monoclonal Antibody [PSH08-47] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

NK1.1 Recombinant Rabbit Monoclonal Antibody [PSH08-47] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse NK1.1 aa 67-223.

Species Reactivity

Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 25 kDa

Positive Control

C57BL/6 mouse thymus tissue.

Conjugation

unconjugated

Clone Number

PSH08-47

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:2,000

Target

Function

Killer cell lectin-like receptor subfamily B, member 1, also known as KLRB1, NKR-P1A or CD161 (cluster of differentiation 161), is a human gene. Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand. Its engagement by NKR-P1 leads to clustering of the latter on the NK cell surface mediating an inhibitory signalling.

Background References

1. Tian D et al. TCRalphabeta(+)NK1.1(-)CD4(-)CD8(-) double-negative T cells inhibit central and peripheral inflammation and ameliorate ischemic stroke in mice. Theranostics. 2023 Jan

2. An JN et al. NK1.1(-) natural killer T cells upregulate interleukin-17 expression in experimental lupus nephritis. Am J Physiol Renal Physiol. 2021 May

Subcellular Location

Membrane.

UNIPROT

SwissProt: P27814 Mouse

Synonyms

CD161 antigen like family member C antibody

CD161 antigen-like family member C antibody

CD161c antibody

Killer cell lectin-like receptor subfamily B member 1C antibody

Klrb1c antibody

Klrb1f antibody

KLRBC_MOUSE antibody

Ly-55c antibody

Ly55c antibody

Lymphocyte antigen 55c antibody

Expand

Images

  • Western blot analysis of NK1.1 on NK1.1 recombinant protein with Rabbit anti-NK1.1 antibody (<a href="/products/HA751222" style="font-weight: bold;text-decoration: underline;">HA751222</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 11 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751222" style="font-weight: bold;text-decoration: underline;">HA751222</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NK1.1 on NK1.1 recombinant protein with Rabbit anti-NK1.1 antibody (HA751222) at 1/1,000 dilution.

    Lysates/proteins at 50 ng/Lane.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 11 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751222) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded C57BL/6 mouse thymus tissue with Rabbit anti-NK1.1 antibody (<a href="/products/HA751222" style="font-weight: bold;text-decoration: underline;">HA751222</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751222" style="font-weight: bold;text-decoration: underline;">HA751222</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded C57BL/6 mouse thymus tissue with Rabbit anti-NK1.1 antibody (HA751222) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751222) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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