Retinoic Acid Receptor alpha/beta/gamma Recombinant Rabbit Monoclonal Antibody [PSH08-74] - BSA and Azide free
Catalog# HA751239
Retinoic Acid Receptor alpha/beta/gamma Recombinant Rabbit Monoclonal Antibody [PSH08-74] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
Retinoic Acid Receptor alpha/beta/gamma Recombinant Rabbit Monoclonal Antibody [PSH08-74] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human RARG aa 155-454.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 50 kDa
Positive Control
SK-Br-3 cell lysate, T-47D cell lysate, A375 cell lysate, HeLa cell lysate.
Conjugation
unconjugated
Clone Number
PSH08-74
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000-1:5,000
Target
Function
The retinoic acid receptor (RAR) is a type of nuclear receptor which can also act as a ligand-activated transcription factor that is activated by both all-trans retinoic acid and 9-cis retinoic acid, retinoid active derivatives of Vitamin A. They are typically found within the nucleus. There are three retinoic acid receptors (RAR), RAR-alpha, RAR-beta, and RAR-gamma, encoded by the RARA, RARB, RARG genes, respectively. Within each RAR subtype there are various isoforms differing in their N-terminal region A. Multiple splice variants have been identified in human RARs: four for RARA, five for RARB, and two for RARG. As with other type II nuclear receptors, RAR heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements known as retinoic acid response elements (RAREs) complexed with corepressor protein. Binding of agonist ligands to RAR results in dissociation of corepressor and recruitment of coactivator protein that, in turn, promotes transcription of the downstream target gene into mRNA and eventually protein. In addition, the expression of RAR genes is under epigenetic regulation by promoter methylation. Both the length and magnitude of the retinoid response is dependent of the degradation of RARs and RXRs through the ubiquitin-proteasome. This degradation can lead to elongation of the DNA transcription through disruption of the initiation complex or to end the response to facilitate further transcriptional programs. RAR receptors are also known to exhibit many retinoid-independent effects as they bind to and regulate other nuclear receptor pathways, such as the estrogen receptor.
Background References
1. Larange A et al. A regulatory circuit controlled by extranuclear and nuclear retinoic acid receptor alpha determines T cell activation and function. Immunity. 2023 Sep
2. Ciancia M et al. Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration. Prog Neurobiol. 2022 May
3. Ji K et al. Retinoic acid receptor gamma is required for proliferation of pancreatic cancer cells. Cell Biol Int. 2023 Jan
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
NR1B1 antibody
Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody
Nuclear receptor subfamily 1 group B member 1 antibody
Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibody
RAR alpha antibody
RAR antibody
RAR-alpha antibody
rara antibody
RARA_HUMAN antibody
RARalpha antibody
ExpandImages
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Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: T-47D cell lysate
Lane 3: A375 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 58 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/1,000 dilution.
Lane 1: Human RARG recombinant protein, 20ng/Lane
Lane 2: Human RARA recombinant protein, 20ng/Lane
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Retinoic Acid Receptor alpha/beta/gamma on different lysates with Rabbit anti-Retinoic Acid Receptor alpha/beta/gamma antibody (HA751239) at 1/5,000 dilution.
Lane 1: Human RARG recombinant protein, 20ng/Lane
Lane 2: Human RARB recombinant protein, 20ng/Lane
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751239) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"