TRIM32 Recombinant Rabbit Monoclonal Antibody [PSH08-78] - BSA and Azide free
Catalog# HA751243
TRIM32 Recombinant Rabbit Monoclonal Antibody [PSH08-78] - BSA and Azide free
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
TRIM32 Recombinant Rabbit Monoclonal Antibody [PSH08-78] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TRIM32 aa 101-653.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 72 kDa
Positive Control
PC-3 cell lysate, HeLa cell lysate, HEK-293 cell lysate, C2C12 cell lysate.
Conjugation
unconjugated
Clone Number
PSH08-78
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Tripartite motif-containing protein 32 is a protein that in humans is encoded by the TRIM32 gene. Since its discovery in 1995, TRIM32 has been shown to be implicated in a number of diverse biological pathways. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
Background References
1. Bawa S et al. TRIM32: A Multifunctional Protein Involved in Muscle Homeostasis, Glucose Metabolism, and Tumorigenesis. Biomolecules. 2021 Mar
2. Jeong SY et al. Tripartite Motif-Containing Protein 32 (TRIM32): What Does It Do for Skeletal Muscle? Cells. 2023 Aug
Subcellular Location
Cytoplasm, Mitochondrion, Endoplasmic reticulum.
UNIPROT
Synonyms
72 kda Tat interacting Protein antibody
72 kDa Tat-interacting protein antibody
BBS11 antibody
E3 ubiquitin-protein ligase TRIM32 antibody
HT2A antibody
LGMD2H antibody
Limb girdle muscular dystrophy 2H (autosomal recessive) antibody
Limb girdle muscular dystrophy 2H antibody
Muscular dystrophy Hutterite type antibody
TAT interactive protein 72KD antibody
ExpandImages
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This data was developed using HA723030, the same antibody clone in a different buffer formulation.
Western blot analysis of TRIM32 on different lysates with Rabbit anti-TRIM32 antibody (HA723030) at 1/2,000 dilution.
Lane 1: PC-3 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: C2C12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723030) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"