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Phosphoserine aminotransferase Recombinant Rabbit Monoclonal Antibody [PSH08-99] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751260

Phosphoserine aminotransferase Recombinant Rabbit Monoclonal Antibody [PSH08-99] - BSA and Azide free

Application

  • WB

  • IHC-P

  • FC

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Phosphoserine aminotransferase Recombinant Rabbit Monoclonal Antibody [PSH08-99] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PSAT1 aa 1-370.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC, IP

Molecular Weight

Predicted band size: 40 kDa

Positive Control

NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, A549 cell lysate, human colon cancer tissue, human kidney tissue, rat kidney tissue, HeLa.

Conjugation

unconjugated

Clone Number

PSH08-99

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate.

Background References

1. Minchenko H O ,Sliusar Y M ,Khikhlo P Y , et al.Knockdown of ERN1 disturbs the expression of phosphoserine aminotransferase 1 and related genes in glioblastoma cells.[J].Archives of biochemistry and biophysics,2024,759110104.

2. Li J ,Wei X ,Sun Y , et al.Phosphoserine aminotransferase deficiency diagnosed by whole-exome sequencing and LC-MS/MS reanalysis: A case report and review of literature.[J].Molecular genetics & genomic medicine,2024,12(4):e2400-e2400.

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: Q9Y617 Human

SwissProt: Q99K85 Mouse

Entrez Gene: 293820 Rat

Synonyms

EC 2.6.1.52 antibody

Endometrial progesterone induced protein antibody

EPIP antibody

MGC1460 antibody

NLS2 antibody

Phosphohydroxythreonine aminotransferase antibody

phosphoserine aminotransferase 1 antibody

Phosphoserine aminotransferase antibody

PSA antibody

PSAT antibody

Expand

Images

  • Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/2,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 2: mouse brain tissue lysate (40 µg/Lane)<br />Lane 3: rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/2,000 dilution.

    Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 2: mouse brain tissue lysate (40 µg/Lane)
    Lane 3: rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751260) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate <br />Lane 3: HepG2 cell lysate <br />Lane 4: K-562 cell lysate <br />Lane 5: A549 cell lysate <br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751260) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phosphoserine aminotransferase antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody (HA751260) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751260) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of HeLa cells labeling Phosphoserine aminotransferase.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling Phosphoserine aminotransferase.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751260, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Phosphoserine aminotransferase was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751260" style="font-weight: bold;text-decoration: underline;">HA751260</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 2 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Phosphoserine aminotransferase was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751260 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751260 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA751260 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA751260 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 2 seconds; ECL: K1801

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