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CYR61 / CCN1 Recombinant Rabbit Monoclonal Antibody [PSH09-12] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751266

CYR61 / CCN1 Recombinant Rabbit Monoclonal Antibody [PSH09-12] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CYR61 / CCN1 Recombinant Rabbit Monoclonal Antibody [PSH09-12] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CCN1 aa 1-381.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 42 kDa

Positive Control

Saos-2 cell lysate, 786-0 cell lysate, Saos-2, human colon tissue, human skin tissue.

Conjugation

unconjugated

Clone Number

PSH09-12

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:2,000

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:50

Target

Function

Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. CYR61 is a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family (CCN intercellular signaling protein). CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

Background References

1. Mooring M et al. Hepatocyte CYR61 polarizes profibrotic macrophages to orchestrate NASH fibrosis. Sci Transl Med. 2023 Sep

2. Song YP et al. Effects of Gestational Arsenic Exposures on Placental and Fetal Development in Mice: The Role of Cyr61 m6A. Environ Health Perspect. 2023 Sep

Subcellular Location

Secreted.

UNIPROT

SwissProt: O00622 Human

Synonyms

3CH61 antibody

CCN 1 antibody

CCN family member 1 antibody

CCN1 antibody

CYR 61 antibody

Cyr61 antibody

CYR61 protein antibody

CYR61_HUMAN antibody

Cysteine rich angiogenic inducer 61 antibody

Cysteine rich heparin binding protein 61 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CYR61 / CCN1 on different lysates with Rabbit anti-CYR61 / CCN1 antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/2,000 dilution.<br /><br />Lane 1: Saos-2 cell lysate<br />Lane 2: 786-0 cell lysate<br />Lane 3: MCF7 cell lysate (negative)<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 27 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CYR61 / CCN1 on different lysates with Rabbit anti-CYR61 / CCN1 antibody (HA751266) at 1/2,000 dilution.

    Lane 1: Saos-2 cell lysate
    Lane 2: 786-0 cell lysate
    Lane 3: MCF7 cell lysate (negative)

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 27 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT),Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of untreated Saos-2 (top, positive) / Saos-2 treated with 10μg/mL BFA overnight (middle, positive) / MCF7 (bottom, negative) labeling CYR61 / CCN1 with Rabbit anti-CYR61 / CCN1 antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/2,000 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYR61 / CCN1 antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT),Relative expression (RE)

    Immunocytochemistry analysis of untreated Saos-2 (top, positive) / Saos-2 treated with 10μg/mL BFA overnight (middle, positive) / MCF7 (bottom, negative) labeling CYR61 / CCN1 with Rabbit anti-CYR61 / CCN1 antibody (HA751266) at 1/2,000 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYR61 / CCN1 antibody (HA751266) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CYR61 / CCN1 antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CYR61 / CCN1 antibody (HA751266) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751266) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CYR61 / CCN1 antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751266" style="font-weight: bold;text-decoration: underline;">HA751266</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-CYR61 / CCN1 antibody (HA751266) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751266) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: colon<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/50

    Application: IF-Tissue

    Species: Human

    Site: colon

    Sample: Paraffin-embedded section

    Antibody concentration: 1/50

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"