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COMMD10 Recombinant Rabbit Monoclonal Antibody [PSH09-13] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751267

COMMD10 Recombinant Rabbit Monoclonal Antibody [PSH09-13] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

COMMD10 Recombinant Rabbit Monoclonal Antibody [PSH09-13] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human COMMD10 aa 1-202.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 23 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, K-562 cell lysate, Human liver tissue lysate, human breast cancer tissue.

Conjugation

unconjugated

Clone Number

PSH09-13

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50

  • IP

  • 1-2μg/sample

Target

Function

May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. May down-regulate activation of NF-kappa-B.

Background References

1. Yang M et al. COMMD10 inhibits HIF1alpha/CP loop to enhance ferroptosis and radiosensitivity by disrupting Cu-Fe balance in hepatocellular carcinoma. J Hepatol. 2022 May

2. Zhao W et al. Comprehensive analysis of COMMD10 as a novel prognostic biomarker for gastric cancer. PeerJ. 2023 Mar

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q9Y6G5 Human

Synonyms

COMM domain-containing protein 10

COMMD10

HSPC305

PTD002

Images

  • Western blot analysis of COMMD10 on different lysates with Rabbit anti-COMMD10 antibody (<a href="/products/HA751267" style="font-weight: bold;text-decoration: underline;">HA751267</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: THP-1 cell lysate (20 µg/Lane)<br />Lane 4: K-562 cell lysate (20 µg/Lane)<br />Lane 5: Human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 23 kDa<br />Observed band size: 25 kDa<br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751267" style="font-weight: bold;text-decoration: underline;">HA751267</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of COMMD10 on different lysates with Rabbit anti-COMMD10 antibody (HA751267) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: THP-1 cell lysate (20 µg/Lane)
    Lane 4: K-562 cell lysate (20 µg/Lane)
    Lane 5: Human liver tissue lysate (40 µg/Lane)

    Predicted band size: 23 kDa
    Observed band size: 25 kDa
    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751267) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-COMMD10 antibody (<a href="/products/HA751267" style="font-weight: bold;text-decoration: underline;">HA751267</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751267" style="font-weight: bold;text-decoration: underline;">HA751267</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-COMMD10 antibody (HA751267) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751267) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"