SLP76 Recombinant Rabbit Monoclonal Antibody [PSH09-22] - BSA and Azide free
Catalog# HA751276
SLP76 Recombinant Rabbit Monoclonal Antibody [PSH09-22] - BSA and Azide free
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WB
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IHC-P
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IF-Cell
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IP
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Human
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HA723080
含抗保成分
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unconjugated
Overview
Product Name
SLP76 Recombinant Rabbit Monoclonal Antibody [PSH09-22] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SLP76 aa 1- 533/533
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell, IP
Molecular Weight
Predicted band size: 60 kDa
Positive Control
THP-1 cell lysate, HL-60 cell lysate, THP-1, human spleen tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
PSH09-22
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:5,000
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IF-Cell
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1:100
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IP
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1-2μg/sample
Target
Function
Lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa), also known as LCP2 or SLP-76, is a signal-transducing adaptor protein expressed in T cells and myeloid cells and is important in the signaling of T-cell receptors (TCRs). As an adaptor protein, SLP-76 does not have catalytic functions, primarily binding other signaling proteins to form larger signaling complexes. It is a key component of the signaling pathways of receptors with immunoreceptor tyrosine-based activation motifs (ITAMs) such as T-cell receptors, its precursors, and receptors for the Fc regions of certain antibodies.SLP-76 is expressed in T-cells and related lymphocytes like natural killer cells.
Background References
1. Lev A, Asleh M, Levy S, Lee YN, Simon AJ,SLP76 Mutation Associated with Combined Immunodeficiency and EBV-Related Lymphoma. J Clin Immunol. 2023 Apr;43(3):625-635. doi: 10.1007/s10875-022-01412-4. Epub 2022 Dec 7. PMID: 36474126.
2. Koretzky GA, Abtahian F, Silverman MA. SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat Rev Immunol. 2006 Jan;6(1):67-78. doi: 10.1038/nri1750. PMID: 16493428.
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
CG8697 antibody
LCP 2 antibody
LCP2 antibody
LCP2_HUMAN antibody
Lymphocyte cytosolic protein 2 antibody
SH2 domain containing leukocyte protein 76 KD antibody
SH2 domain containing leukocyte protein of 76kD antibody
SH2 domain containing leukocyte protein of 76kDa antibody
SH2 domain-containing leukocyte protein of 76 kDa antibody
SLP 76 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of SLP76 on different lysates with Rabbit anti-SLP76 antibody (HA751276) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: HL-60 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751276) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling SLP76 with Rabbit anti-SLP76 antibody (HA751276) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLP76 antibody (HA751276) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-SLP76 antibody (HA751276) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751276) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SLP76 antibody (HA751276) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751276) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
SLP76 was immunoprecipitated from 0.2 mg HL-60 cell lysate with HA751276 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751276 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HL-60 cell lysate (input)
Lane 2: HA751276 IP in HL-60 cell lysate
Lane 3: Rabbit IgG instead of HA751276 in HL-60 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 14 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
SLP76 Recombinant Rabbit Monoclonal Antibody [PSH09-22]
Application: WB,IHC-P,IF-Cell,IP
Reactivity: Human
Conjugate: unconjugated
