TGF beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-26] - BSA and Azide free
Catalog# HA751280
TGF beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-26] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TGF beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-26] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TGF beta 3 aa 1-300.
Product Specificity
This antibody does not cross-react with TGFB1 and TGFB2.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 47 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, PC-12, human ovary tissue, mouse fallopian tissue, mouse ovary tissue, rat fallopian tissue, rat ovary tissue.
Conjugation
unconjugated
Clone Number
PSH09-26
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Transforming growth factor beta-3 is a protein that in humans is encoded by the TGFB3 gene. It is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily, which includes the TGF-β family, Bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs), inhibins and activins. TGF-β3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-β3, mammals develop a deformity known as a cleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-β3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin.
Background References
1. Watanabe M et al. TGF-beta-3 Induces Different Effects from TGF-beta-1 and -2 on Cellular Metabolism and the Spatial Properties of the Human Trabecular Meshwork Cells. Int J Mol Sci. 2023 Feb
2. Cetik RM et al. Evaluation of the Effects of Transforming Growth Factor-Beta 3 (TGF-beta3) Loaded Nanoparticles on Healing in a Rat Achilles Tendon Injury Model. Am J Sports Med. 2022 Mar
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Synonyms
ARVD antibody
ARVD1 antibody
FLJ16571 antibody
LDS5 antibody
MGC105479 antibody
MGC118722 antibody
prepro-transforming growth factor beta-3 antibody
RNHF antibody
TGF beta 3 antibody
TGF beta3 antibody
ExpandImages
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This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Western blot analysis of TGF beta 3 on different lysates with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 60 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723084) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Western blot analysis of TGF beta 3 on different lysates with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/2,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Mouse liver tissue lysate
Lane 4: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 60 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723084) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of PC-12 cells labeling TGF beta 3 with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse fallopian tissue with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat fallopian tissue with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723084, the same antibody clone in a different buffer formulation.
Western blot analysis of TGF beta 3 on different lysates with Rabbit anti-TGF beta 3 antibody (HA723084) at 1/5,000 dilution.
Lane 1: His-tagged Human TGFB3 recombinant protein
Lane 2: His-tagged Human TGFB2 recombinant protein
Lysates/proteins at 50 ng/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723084) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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