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Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [PSH09-32] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751286

Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [PSH09-32] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [PSH09-32] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CKB aa 1-381.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 43 kDa

Positive Control

HeLa cell lysate, SH-SY5Y cell lysate, Y79 cell lysate, C6 cell lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, SH-SY5Y.

Conjugation

unconjugated

Clone Number

PSH09-32

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene, CK-BB, consists of a homodimer of two identical brain-type CK-B subunits. BB-CK is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CK-BB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer (CK-BB) in brain and smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone, etc. In heart, a heterodimer (CK-MB) shahil consisting of one CK-B brain-type CK subunit and one CK-M muscle-type CK subunit is prominently expressed. The encoded CK-BB and CK-MB proteins are members of the ATP:guanido phosphotransferase protein family.

Background References

1. Zhou F et al. CKB affects human osteosarcoma progression by regulating the p53 pathway. Am J Cancer Res. 2022 Oct

2. Zhou FL et al. Effects of plumbagin on proliferation and apoptosis of human hepatocellular carcinoma cells based on CKB/p53 signaling pathway. Zhongguo Zhong Yao Za Zhi. 2024 May

Subcellular Location

Cytoplasm, cytosol, Mitochondrion, Cell membrane.

UNIPROT

SwissProt: P12277 Human

SwissProt: Q04447 Mouse

SwissProt: P07335 Rat

Synonyms

B CK antibody

B-CK antibody

BB-CK antibody

BCK antibody

Brain creatine kinase antibody

Ckb antibody

CKBB antibody

Creatine kinase B antibody

Creatine kinase B chain antibody

Creatine kinase B type antibody

Expand

Images

  • This data was developed using <a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (<a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: SH-SY5Y cell lysate<br />Lane 3: Y79 cell lysate<br />Lane 4: C6 cell lysate<br />Lane 5: Mouse cerebellum tissue lysate<br />Lane 6: Rat cerebellum tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723090, the same antibody clone in a different buffer formulation.
    Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: Y79 cell lysate
    Lane 4: C6 cell lysate
    Lane 5: Mouse cerebellum tissue lysate
    Lane 6: Rat cerebellum tissue lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 45 kDa

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723090) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>, the same antibody clone in a different buffer formulation.<br />Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody (<a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>) at 1/2,000 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody (<a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    This data was developed using HA723090, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • This data was developed using <a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>, the same antibody clone in a different buffer formulation.<br />Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA723090" style="font-weight: bold;text-decoration: underline;">HA723090</a>, 1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    This data was developed using HA723090, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA723090, 1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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