Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [PSH09-32] - BSA and Azide free
Catalog# HA751286
Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [PSH09-32] - BSA and Azide free
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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HA723090
含抗保成分
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unconjugated
Overview
Product Name
Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [PSH09-32] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CKB aa 1-381.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 43 kDa
Positive Control
HeLa cell lysate, SH-SY5Y cell lysate, Y79 cell lysate, C6 cell lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, SH-SY5Y.
Conjugation
unconjugated
Clone Number
PSH09-32
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:2,000
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FC
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1:200-1:1,000
Target
Function
Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene, CK-BB, consists of a homodimer of two identical brain-type CK-B subunits. BB-CK is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CK-BB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer (CK-BB) in brain and smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone, etc. In heart, a heterodimer (CK-MB) shahil consisting of one CK-B brain-type CK subunit and one CK-M muscle-type CK subunit is prominently expressed. The encoded CK-BB and CK-MB proteins are members of the ATP:guanido phosphotransferase protein family.
Background References
1. Zhou F et al. CKB affects human osteosarcoma progression by regulating the p53 pathway. Am J Cancer Res. 2022 Oct
2. Zhou FL et al. Effects of plumbagin on proliferation and apoptosis of human hepatocellular carcinoma cells based on CKB/p53 signaling pathway. Zhongguo Zhong Yao Za Zhi. 2024 May
Subcellular Location
Cytoplasm, cytosol, Mitochondrion, Cell membrane.
Synonyms
B CK antibody
B-CK antibody
BB-CK antibody
BCK antibody
Brain creatine kinase antibody
Ckb antibody
CKBB antibody
Creatine kinase B antibody
Creatine kinase B chain antibody
Creatine kinase B type antibody
ExpandImages
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Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (HA751286) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: Y79 cell lysate
Lane 4: C6 cell lysate
Lane 5: Mouse cerebellum tissue lysate
Lane 6: Rat cerebellum tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 45 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751286) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody (HA751286) at 1/2,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody (HA751286) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751286, 1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"