eIF3g Recombinant Rabbit Monoclonal Antibody [PSH09-41] - BSA and Azide free
Catalog# HA751288
eIF3g Recombinant Rabbit Monoclonal Antibody [PSH09-41] - BSA and Azide free
-
WB
-
IF-Cell
-
FC
-
IP
-
Human
-
unconjugated
Overview
Product Name
eIF3g Recombinant Rabbit Monoclonal Antibody [PSH09-41] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human eIF3g aa 1-320.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC, IP
Target Molecular Weight
Predicted band size: 36 kDa
Positive Control
K-562 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, HeLa.
Conjugation
unconjugated
Clone Number
PSH09-41
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:25,000
-
IF-Cell
-
1:100
-
FC
-
1:1,000
-
IP
-
1-2μg/sample
Target
Function
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Background References
1. Chang J et al. An interaction between eIF4A3 and eIF3g drives the internal initiation of translation. Nucleic Acids Res. 2023 Nov
2. Zhang X et al. Long non-coding RNA LINC02446 suppresses the proliferation and metastasis of bladder cancer cells by binding with EIF3G and regulating the mTOR signalling pathway. Cancer Gene Ther. 2021 Dec
Subcellular Location
Cytoplasm, Nucleus, perinuclear region.
UNIPROT
Synonyms
44kDa antibody
Anti EIF3S4 antibody
eIF 3 delta antibody
eIF 3 RNA binding subunit antibody
eIF-3 RNA-binding subunit antibody
eIF-3-delta antibody
eIF3 delta antibody
eIF3 p42 antibody
eIF3 p44 antibody
eIF3g antibody
ExpandImages
-
Western blot analysis of eIF3g on different lysates with Rabbit anti-eIF3g antibody (HA751288) at 1/25,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 45 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751288) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling eIF3g with Rabbit anti-eIF3g antibody (HA751288) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF3g antibody (HA751288) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling eIF3g.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751288, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
eIF3g was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA751288 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751288 at 1/5,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate (input)
Lane 2: HA751288 IP in SH-SY5Y cell lysate
Lane 3: Rabbit IgG instead of HA751288 in SH-SY5Y cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 12 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"