Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Recombinant Rabbit Monoclonal Antibody [PSH09-43] - BSA and Azide free
Catalog# HA751290
Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Recombinant Rabbit Monoclonal Antibody [PSH09-43] - BSA and Azide free
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WB
-
IF-Cell
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Human
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Mouse
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Rat
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HA723105
含抗保成分
-
unconjugated
Overview
Product Name
Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Recombinant Rabbit Monoclonal Antibody [PSH09-43] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr394 of human LCK.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 58 kDa
Positive Control
COLO205 cell lysate, TT cell lysate, Ramos serum starved for 16 hours cell lysate, Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes.
Conjugation
unconjugated
Clone Number
PSH09-43
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
Target
Function
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells. Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active.
Background References
暂无
Subcellular Location
Cell membrane, Cytoplasm, cytosol.
UNIPROT
Synonyms
AA407514 antibody
EC 2.7.10.2 antibody
FLJ26625 antibody
Hck 2 antibody
JTK 8 antibody
JTK8 antibody
Lck/Yes related novel protein tyrosine kinase antibody
LYN antibody
LYN proto oncogene, Src family tyrosine kinase antibody
LYN_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT),Relative expression (RE)
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/2,000 dilution.
Lane 1: COLO205 cell lysate
Lane 2: TT cell lysate (negative)
Lane 3: Ramos serum starved for 16 hours cell lysate
Lane 4: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate
Lane 5: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 23 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751290) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/2,000 dilution.
Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate (20 µg/Lane)
Lane 3: PC-12 cell lysate (20 µg/Lane)
Lane 4: PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate (20 µg/Lane)
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751290) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody (HA751290) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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