Phospho-AKT2 (S474) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-46] (HA751293)
Catalog# HA751293
Rabbit Monoclonal to Phospho-AKT2 (S474)
Tested for
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WB
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-AKT2 (S474) on different lysates with Rabbit anti-Phospho-AKT2 (S474) antibody (HA751293) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: HEK-293 treated with 50μM LY294002 for 6 hours cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate
Lane 7: C6 cell lysate
Lane 8: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: Lane 1-6: 3 minutes; Lane 7-8: 12 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751293) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-AKT2 (S474) on different lysates with Rabbit anti-Phospho-AKT2 (S474) antibody (HA751293) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: HEK-293 treated with 50μM LY294002 for 6 hours cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: NIH/3T3 treated with 100ng/mL PDGF for 1 hour cell lysate
Lane 7: C6 cell lysate
Lane 8: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: Lane 1-6: 3 minutes; Lane 7-8: 12 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751293) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.