SEC61B Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-48] (HA751295)
Catalog# HA751295
Rabbit Monoclonal to SEC61B
Tested for
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WB
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IHC-P
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IF-Cell
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IP
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SEC61B on different lysates with Rabbit anti-SEC61B antibody (HA751295) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 10 kDa
Observed band size: 14 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751295) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 10 kDa
Observed band size: 14 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751295) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.