SOCS1 Recombinant Rabbit Monoclonal Antibody [PSH09-68] - BSA and Azide free
Catalog# HA751310
SOCS1 Recombinant Rabbit Monoclonal Antibody [PSH09-68] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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Green monkey
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HA723133
含抗保成分
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unconjugated
Overview
Product Name
SOCS1 Recombinant Rabbit Monoclonal Antibody [PSH09-68] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SOCS1 aa 1-211.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 24 kDa
Positive Control
K-562 cell lysate, HDLM-2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, A20 cell lysate, Rat thymus tissue lysate.
Conjugation
unconjugated
Clone Number
PSH09-68
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Suppressor of cytokine signaling 1 is a protein that in humans is encoded by the SOCS1 gene. SOCS1 orthologs have been identified in several mammals for which complete genome data are available. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signalling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3, erythropoietin (EPO), GM-CSF, and interferon-gamma (IFN-γ). The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-γ action, which is required for normal postnatal growth and survival. Several recent viral studies have shown that viral genes, such as Tax gene product (Tax), encoded by HTLV-1, could hijack SOCS1 to inhibit host antiviral pathways, as a strategy to evade host immunity.
Background References
1. Schlabach MR et al. Rational design of a SOCS1-edited tumor-infiltrating lymphocyte therapy using CRISPR/Cas9 screens. J Clin Invest. 2023 Dec
2. Sutra Del Galy A et al. In vivo genome-wide CRISPR screens identify SOCS1 as intrinsic checkpoint of CD4(+) T(H)1 cell response. Sci Immunol. 2021 Dec
Subcellular Location
Nucleus, Cytoplasmic vesicle.
Synonyms
CIS1 antibody
CISH 1 antibody
CISH1 antibody
Cytokine inducible SH2 protein 1 antibody
JAB antibody
JAK binding protein antibody
JAK-binding protein antibody
Janus kinase binding protein antibody
SOCS 1 antibody
SOCS-1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of SOCS1 on different lysates with Rabbit anti-SOCS1 antibody (HA751310) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (30 µg/Lane)
Lane 2: HDLM-2 cell lysate (30 µg/Lane)
Lane 3: 293T cell lysate (negative) (30 µg/Lane)
Lane 4: COS-1 cell lysate (30 µg/Lane)
Lane 5: NIH/3T3 cell lysate (30 µg/Lane)
Lane 6: A20 cell lysate (low expression) (30 µg/Lane)
Lane 7: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751310) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
SOCS1 Rabbit Polyclonal Antibody
Application: IF-Cell,WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
