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Phospho-STING (S366) Recombinant Rabbit Monoclonal Antibody [PSH09-72] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751314

Phospho-STING (S366) Recombinant Rabbit Monoclonal Antibody [PSH09-72] - BSA and Azide free

Application

  • WB

  • Dot Blot

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Phospho-STING (S366) Recombinant Rabbit Monoclonal Antibody [PSH09-72] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser366 of Human STING.

Species Reactivity

Human

Validated Applications

WB, Dot Blot, IP

Target Molecular Weight

Predicted band size: 42 kDa

Positive Control

THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH09-72

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • Dot Blot

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Stimulator of interferon genes (STING), also known as transmembrane protein 173 (TMEM173) and MPYS/MITA/ERIS is a protein that in humans is encoded by the STING1 gene. STING plays an important role in innate immunity. STING induces type I interferon production when cells are infected with intracellular pathogens, such as viruses, mycobacteria and intracellular parasites.Type I interferon, mediated by STING, protects infected cells and nearby cells from local infection by binding to the same cell that secretes it (autocrine signaling) and nearby cells (paracrine signaling.) It thus plays an important role, for instance, in controlling norovirus infection. STING works as both a direct cytosolic DNA sensor (CDS) and an adaptor protein in Type I interferon signaling through different molecular mechanisms. It has been shown to activate downstream transcription factors STAT6 and IRF3 through TBK1, which are responsible for antiviral response and innate immune response against intracellular pathogen.

Background References

1. Liu K et al. Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy. Autophagy. 2022 Apr

2. Zhang R et al. STING1 in Different Organelles: Location Dictates Function. Front Immunol. 2022 Mar

Subcellular Location

Endoplasmic reticulum membrane, Cytoplasm, perinuclear region, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Mitochondrion outer membrane, Cell membrane.

UNIPROT

SwissProt: Q86WV6 Human

SwissProt: Q3TBT3 Mouse

SwissProt: F1M391 Rat

Synonyms

endoplasmic reticulum IFN stimulator antibody

Endoplasmic reticulum interferon stimulator antibody

ERIS antibody

FLJ38577 antibody

hMITA antibody

hSTING antibody

Mediator of IRF3 activation antibody

MITA antibody

Mitochondrial mediator of IRF3 activation antibody

MPYS antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-STING (S366) on different lysates with Rabbit anti-Phospho-STING (S366) antibody (<a href="/products/HA751314" style="font-weight: bold;text-decoration: underline;">HA751314</a>) at 1/2,000 dilution.<br /><br />Lane 1: THP-1 treated with 80nM TPA for 16 hours cell lysate<br />Lane 2: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate<br />Lane 3: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751314" style="font-weight: bold;text-decoration: underline;">HA751314</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-STING (S366) on different lysates with Rabbit anti-Phospho-STING (S366) antibody (HA751314) at 1/2,000 dilution.

    Lane 1: THP-1 treated with 80nM TPA for 16 hours cell lysate
    Lane 2: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate
    Lane 3: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751314) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Dot blot analysis of Phospho-STING (S366) on different peptides with Rabbit anti-Phospho-STING (S366) antibody (<a href="/products/HA751314" style="font-weight: bold;text-decoration: underline;">HA751314</a>) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution for 1 hour at room temperature.<br /><br />Lane 1: Unmodified STING peptide (negative)<br />Lane 2: Phospho-STING (S366) peptide (positive)<br /><br />Proteins loading: 100ng, 50ng, 10ng, 5ng;<br /><br />Blocking and dilution buffer: 5% NFDM/TBST;<br /><br />Exposure time: 1 minute; ECL: K1801.

    Dot blot analysis of Phospho-STING (S366) on different peptides with Rabbit anti-Phospho-STING (S366) antibody (HA751314) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.

    Lane 1: Unmodified STING peptide (negative)
    Lane 2: Phospho-STING (S366) peptide (positive)

    Proteins loading: 100ng, 50ng, 10ng, 5ng;

    Blocking and dilution buffer: 5% NFDM/TBST;

    Exposure time: 1 minute; ECL: K1801.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Phospho-STING (S366) was immunoprecipitated from 0.2 mg THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate with <a href="/products/HA751314" style="font-weight: bold;text-decoration: underline;">HA751314</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751314" style="font-weight: bold;text-decoration: underline;">HA751314</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate (input)<br />Lane 2: <a href="/products/HA751314" style="font-weight: bold;text-decoration: underline;">HA751314</a> IP in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751314" style="font-weight: bold;text-decoration: underline;">HA751314</a> in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 10 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    ☑ Cell treatment (CT)

    Phospho-STING (S366) was immunoprecipitated from 0.2 mg THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate with HA751314 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751314 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate (input)
    Lane 2: HA751314 IP in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate
    Lane 3: Rabbit IgG instead of HA751314 in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 10 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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