Phospho-RICTOR (T1135) Recombinant Rabbit Monoclonal Antibody [PSH09-92] - BSA and Azide free
Catalog# HA751323
Phospho-RICTOR (T1135) Recombinant Rabbit Monoclonal Antibody [PSH09-92] - BSA and Azide free
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
Phospho-RICTOR (T1135) Recombinant Rabbit Monoclonal Antibody [PSH09-92] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr1135 of Human RICTOR.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 192 kDa
Positive Control
HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate.
Conjugation
unconjugated
Clone Number
PSH09-92
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR) is a protein that in humans is encoded by the RICTOR gene. RICTOR and mTOR are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth. RICTOR is a subunit of the mammalian target of rapamycin complex 2 (mTORC2) which contains mTOR, GβL, RICTOR (this protein) and mSIN1. The mammalian target of rapamycin (mTOR) is a highly conserved Ser/Thr kinase that regulates cell growth and proliferation. mTOR may exist as mTOR complex 1 (mTORC1) or mTOR complex 2 (mTORC2). RICTOR is a key component of mTORC2, which, unlike mTORC1, is not directly inhibited by rapamycin. mTORC2, and RICTOR, specifically, has been shown to phosphorylate Akt/protein kinase B (PKB) on SER473. This phosphorylation activates Akt/PKB, where deregulation of Akt/PKB has been implicated in cancer and diabetes. RICTOR and mTORC2 have been shown to play an essential role in embryonic growth and development, perhaps due to the control that mTORC2 exerts on actin cytoskeleton organization. Diseases associated with mutation in the RICTOR gene include foramen magnum meningioma and syringomyelia. Akt/PMB activation is also involved in glucose metabolism and activation of Akt by RICTOR has been shown to mediate glucose and lipid metabolism. Therefore, the influence of RICTOR and mTORC2 on Akt signaling has been associated with insulin resistance and type 2 diabetes.
Background References
1. Ramaiah MJ. et. al. mTOR-Rictor-EGFR axis in oncogenesis and diagnosis of glioblastoma multiforme. Mol Biol Rep. 2021 May
2. Xu S. et. al. Rictor Is a Novel Regulator of TRAF6/TRAF3 in Osteoclasts. J Bone Miner Res. 2021 Oct
Subcellular Location
Cytosol, Golgi apparatus.
Synonyms
AVO3 antibody
AVO3 homolog antibody
DKFZp686B11164 antibody
hAVO3 antibody
KIAA1999 antibody
Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
mAVO3 antibody
MGC39830 antibody
PIA antibody
Pianissimo antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA751323) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate
Lane 3: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 192 kDa
Observed band size: 240 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751323) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA751323) at 1/2,000 dilution.
Lane 1: C2C12 starved overnight cell lysate
Lane 2: C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 192 kDa
Observed band size: 240 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751323) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"