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Phospho-RICTOR (T1135) Recombinant Rabbit Monoclonal Antibody [PSH09-92] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751323

Phospho-RICTOR (T1135) Recombinant Rabbit Monoclonal Antibody [PSH09-92] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Phospho-RICTOR (T1135) Recombinant Rabbit Monoclonal Antibody [PSH09-92] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr1135 of Human RICTOR.

Species Reactivity

Human, Mouse

Validated Applications

WB

Target Molecular Weight

Predicted band size: 192 kDa

Positive Control

HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate.

Conjugation

unconjugated

Clone Number

PSH09-92

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR) is a protein that in humans is encoded by the RICTOR gene. RICTOR and mTOR are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth. RICTOR is a subunit of the mammalian target of rapamycin complex 2 (mTORC2) which contains mTOR, GβL, RICTOR (this protein) and mSIN1. The mammalian target of rapamycin (mTOR) is a highly conserved Ser/Thr kinase that regulates cell growth and proliferation. mTOR may exist as mTOR complex 1 (mTORC1) or mTOR complex 2 (mTORC2). RICTOR is a key component of mTORC2, which, unlike mTORC1, is not directly inhibited by rapamycin. mTORC2, and RICTOR, specifically, has been shown to phosphorylate Akt/protein kinase B (PKB) on SER473. This phosphorylation activates Akt/PKB, where deregulation of Akt/PKB has been implicated in cancer and diabetes. RICTOR and mTORC2 have been shown to play an essential role in embryonic growth and development, perhaps due to the control that mTORC2 exerts on actin cytoskeleton organization. Diseases associated with mutation in the RICTOR gene include foramen magnum meningioma and syringomyelia. Akt/PMB activation is also involved in glucose metabolism and activation of Akt by RICTOR has been shown to mediate glucose and lipid metabolism. Therefore, the influence of RICTOR and mTORC2 on Akt signaling has been associated with insulin resistance and type 2 diabetes.

Background References

1. Ramaiah MJ. et. al. mTOR-Rictor-EGFR axis in oncogenesis and diagnosis of glioblastoma multiforme. Mol Biol Rep. 2021 May

2. Xu S. et. al. Rictor Is a Novel Regulator of TRAF6/TRAF3 in Osteoclasts. J Bone Miner Res. 2021 Oct

Subcellular Location

Cytosol, Golgi apparatus.

UNIPROT

SwissProt: Q6R327 Human

SwissProt: Q6QI06 Mouse

Synonyms

AVO3 antibody

AVO3 homolog antibody

DKFZp686B11164 antibody

hAVO3 antibody

KIAA1999 antibody

Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody

mAVO3 antibody

MGC39830 antibody

PIA antibody

Pianissimo antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />This data was developed using <a href="/products/HA723161" style="font-weight: bold;text-decoration: underline;">HA723161</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (<a href="/products/HA723161" style="font-weight: bold;text-decoration: underline;">HA723161</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate<br />Lane 3: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 192 kDa<br />Observed band size: 240 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723161" style="font-weight: bold;text-decoration: underline;">HA723161</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    This data was developed using HA723161, the same antibody clone in a different buffer formulation.
    Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate
    Lane 3: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 192 kDa
    Observed band size: 240 kDa

    Exposure time: 59 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />This data was developed using <a href="/products/HA723161" style="font-weight: bold;text-decoration: underline;">HA723161</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (<a href="/products/HA723161" style="font-weight: bold;text-decoration: underline;">HA723161</a>) at 1/2,000 dilution.<br /><br />Lane 1: C2C12 starved overnight cell lysate<br />Lane 2: C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 192 kDa<br />Observed band size: 240 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723161" style="font-weight: bold;text-decoration: underline;">HA723161</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    This data was developed using HA723161, the same antibody clone in a different buffer formulation.
    Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution.

    Lane 1: C2C12 starved overnight cell lysate
    Lane 2: C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 192 kDa
    Observed band size: 240 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"