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LPCAT3 Recombinant Rabbit Monoclonal Antibody [PSH10-02] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751332

LPCAT3 Recombinant Rabbit Monoclonal Antibody [PSH10-02] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

LPCAT3 Recombinant Rabbit Monoclonal Antibody [PSH10-02] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human LPCAT3 aa 1-363.

Species Reactivity

Human, Green monkey

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 56 kDa

Positive Control

HT-29 cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, HeLa.

Conjugation

unconjugated

Clone Number

PSH10-02

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

Lysophospholipid acyltransferase 5 is an enzyme that in humans is encoded by the LPCAT3 gene. It is homologous to other membrane-bound O-acyltransferases. Based on the crystalographic and cryo-EM studies of its homolog in chicken (cLPCAT3), humane MBOAT5 has a typical MBOAT folding as other members such as SOAT1 and DGAT1, and the transmembrane helices hold a "T"-shape reaction chamber allowing the co-occupancy of a lysophosphatidylcholine (lysoPC) and a long polyunsaturated acyl-CoA, such as arachidonic acyl CoA. With the assistance of catalytic residues H374 and N338, the acyl chain could be transferred from the acyl CoA to the sn-2 position of lysoPC, thereby generating a new, polyunsaturated phospholipid. Inhibition of LPCAT3 has been found to alter the cellular lipidome and is partially protective against ferroptosis.

Background References

1. Cui J et al. LPCAT3 Is Transcriptionally Regulated by YAP/ZEB/EP300 and Collaborates with ACSL4 and YAP to Determine Ferroptosis Sensitivity. Antioxid Redox Signal. 2023 Sep

2. Tao Q et al. Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-gamma-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors. J Immunother Cancer. 2024 Mar

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q6P1A2 Human

Synonyms

1-acylglycerophosphocholine O-acyltransferase antibody

1-acylglycerophosphoserine O-acyltransferase antibody

LPCAT antibody

Lpcat3 antibody

LPLAT 5 antibody

LPSAT antibody

Lyso-PC acyltransferase 3 antibody

Lyso-PC acyltransferase antibody

Lyso-PS acyltransferase antibody

Lysophosphatidylcholine acyltransferase 3 antibody

Expand

Images

  • Western blot analysis of LPCAT3 on different lysates with Rabbit anti-LPCAT3 antibody (<a href="/products/HA751332" style="font-weight: bold;text-decoration: underline;">HA751332</a>) at 1/2,000 dilution.<br /><br />Lane 1: HT-29 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751332" style="font-weight: bold;text-decoration: underline;">HA751332</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LPCAT3 on different lysates with Rabbit anti-LPCAT3 antibody (HA751332) at 1/2,000 dilution.

    Lane 1: HT-29 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 45 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751332) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling LPCAT3 with Rabbit anti-LPCAT3 antibody (<a href="/products/HA751332" style="font-weight: bold;text-decoration: underline;">HA751332</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LPCAT3 antibody (<a href="/products/HA751332" style="font-weight: bold;text-decoration: underline;">HA751332</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling LPCAT3 with Rabbit anti-LPCAT3 antibody (HA751332) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LPCAT3 antibody (HA751332) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"