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Synapsin I + II Recombinant Rabbit Monoclonal Antibody [PSH10-29] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751346

Synapsin I + II Recombinant Rabbit Monoclonal Antibody [PSH10-29] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-Fr

  • IHC-P

  • IP

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

Synapsin I + II Recombinant Rabbit Monoclonal Antibody [PSH10-29] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Synapsin I aa 1-705.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IHC-Fr, IHC-P, IP, IF-Tissue

Target Molecular Weight

Predicted band size: 74 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate, mouse primary neuronal, human brain tissue, mouse brain tissue, mouse retina tissue, rat brain tissue, rat retina tissue, IMR-32 cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate.

Conjugation

unconjugated

Clone Number

PSH10-29

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:25,000

  • IF-Cell

  • 1:2,500

  • IHC-Fr

  • 1:2,500

  • IHC-P

  • 1:1,000-1:5,000

  • IP

  • 1-2μg/sample

  • IF-Tissue

  • 1:200-1:1,000

Target

Function

Synapsin I, is the collective name for Synapsin Ia and Synapsin Ib, two nearly identical phosphoproteins that in humans are encoded by the SYN1 gene. In its phosphorylated form, Synapsin I may also be referred to as phosphosynaspin I. Synapsin I is the first of the proteins in the synapsin family of phosphoproteins in the synaptic vesicles present in the central and peripheral nervous systems. Synapsin Ia and Ib are close in length and almost the same in make up, however, Synapsin Ib stops short of the last segment of the C-terminal in the amino acid sequence found in Synapsin Ia. Synapsin II is the collective name for synapsin IIa and synapsin IIb, two nearly identical phosphoproteins in the synapsin family that in humans are encoded by the SYN2 gene. Synapsins associate as endogenous substrates to the surface of synaptic vesicles and act as key modulators in neurotransmitter release across the presynaptic membrane of axonal neurons in the nervous system.

Background References

1. Forte N et al. Synapsin I Synchronizes GABA Release in Distinct Interneuron Subpopulations. Cereb Cortex. 2020 Mar

2. Schwark R et al. Synapsin II Directly Suppresses Epileptic Seizures In Vivo. Brain Sci. 2022 Feb

Subcellular Location

Synapse, Golgi apparatus, Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.

UNIPROT

SwissProt: P17600 Human

SwissProt: Q92777 Human

SwissProt: O88935 Mouse

SwissProt: Q64332 Mouse

SwissProt: P09951 Rat

SwissProt: Q63537 Rat

Synonyms

Brain protein 4.1 antibody

SYN 1 antibody

SYN 1a antibody

SYN 1b antibody

SYN I antibody

SYN1 antibody

SYN1_HUMAN antibody

SYN1a antibody

SYN1b antibody

Synapsin 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/25,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse lung tissue lysate (negative)<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat lung tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 50-74 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/25,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse lung tissue lysate (negative)
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat lung tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 50-74 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751346) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/25,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse lung tissue lysate (negative)<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat lung tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 50-74 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/25,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse lung tissue lysate (negative)
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat lung tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 50-74 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751346) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebellum<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>, 1/2,500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA751346, 1/2,500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Hippocampus<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>, 1/2,500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Hippocampus
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA751346, 1/2,500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Retina<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>, 1/2,500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Retina
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA751346, 1/2,500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Rat<br />Tissue: Cerebral cortex<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>, 1/2,500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Cerebral cortex
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA751346, 1/2,500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751346) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human lung tissue (negative) with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human lung tissue (negative) with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751346) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751346) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751346) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751346) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751346) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Synapsin I + II was immunoprecipitated from 0.2 mg mouse brain tissue lysate with <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a> at 1/5,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Mouse brain tissue lysate (input)<br />Lane 2: <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a> IP in mouse brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a> in mouse brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Synapsin I + II was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751346 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751346 at 1/5,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Mouse brain tissue lysate (input)
    Lane 2: HA751346 IP in mouse brain tissue lysate
    Lane 3: Rabbit IgG instead of HA751346 in mouse brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 seconds; ECL: K1801

  • Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/25,000 dilution.<br /><br />Lane 1: IMR-32 cell lysate<br />Lane 2: U-87 MG cell lysate<br />Lane 3: Neuro-2a cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 74/55 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751346" style="font-weight: bold;text-decoration: underline;">HA751346</a>) at 1/25,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (HA751346) at 1/25,000 dilution.

    Lane 1: IMR-32 cell lysate
    Lane 2: U-87 MG cell lysate
    Lane 3: Neuro-2a cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 74/55 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751346) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"