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FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751347

FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FOXM1 aa 1-400.

Species Reactivity

Human, Mouse

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 84 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, LO2 cell lysate, Mouse testis tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-30

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Forkhead box protein M1 is a protein that in humans is encoded by the FOXM1 gene. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases. FOXM1-null mouse embryos were neonatal lethal as a result of the development of polyploid cardiomyocytes and hepatocytes, highlighting the role of FOXM1 in mitotic division. More recently a study using transgenic/knockout mouse embryonic fibroblasts and human osteosarcoma cells (U2OS) has shown that FOXM1 regulates expression of a large array of G2/M-specific genes, such as Plk1, cyclin B2, Nek2 and CENPF, and plays an important role in maintenance of chromosomal segregation and genomic stability.

Background References

1. Khan MA et al. FOXM1: A small fox that makes more tracks for cancer progression and metastasis. Semin Cancer Biol. 2023 Jul

2. Zhang Z et al. FOXM1: Functional Roles of FOXM1 in Non-Malignant Diseases. Biomolecules. 2023 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q08050 Human

SwissProt: O08696 Mouse

Synonyms

FKHL16 antibody

Forkhead box M1 antibody

Forkhead box protein M1 antibody

forkhead like 16 antibody

Forkhead-related protein FKHL16 antibody

FOX M1 antibody

Foxm1 antibody

FOXM1_HUMAN antibody

FOXM1B antibody

Hepatocyte nuclear factor 3 forkhead homolog 11 antibody

Expand

Images

  • Western blot analysis of FOXM1 on different lysates with Rabbit anti-FOXM1 antibody (<a href="/products/HA751347" style="font-weight: bold;text-decoration: underline;">HA751347</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 cell lysate (20 µg/Lane)<br />Lane 3: HT-29 cell lysate (20 µg/Lane)<br />Lane 4: LO2 cell lysate (20 µg/Lane)<br />Lane 5: Mouse testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 84 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751347" style="font-weight: bold;text-decoration: underline;">HA751347</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FOXM1 on different lysates with Rabbit anti-FOXM1 antibody (HA751347) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HEK-293 cell lysate (20 µg/Lane)
    Lane 3: HT-29 cell lysate (20 µg/Lane)
    Lane 4: LO2 cell lysate (20 µg/Lane)
    Lane 5: Mouse testis tissue lysate (40 µg/Lane)

    Predicted band size: 84 kDa
    Observed band size: 110 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751347) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • FOXM1 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA751347" style="font-weight: bold;text-decoration: underline;">HA751347</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751347" style="font-weight: bold;text-decoration: underline;">HA751347</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA751347" style="font-weight: bold;text-decoration: underline;">HA751347</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751347" style="font-weight: bold;text-decoration: underline;">HA751347</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 4 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    FOXM1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751347 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751347 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA751347 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA751347 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 4 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"