FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30] - BSA and Azide free
Catalog# HA751347
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30] - BSA and Azide free
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WB
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IP
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Human
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Mouse
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unconjugated
Overview
Product Name
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FOXM1 aa 1-400.
Species Reactivity
Human, Mouse
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 84 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, LO2 cell lysate, Mouse testis tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-30
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Forkhead box protein M1 is a protein that in humans is encoded by the FOXM1 gene. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases. FOXM1-null mouse embryos were neonatal lethal as a result of the development of polyploid cardiomyocytes and hepatocytes, highlighting the role of FOXM1 in mitotic division.
Background References
1. Khan MA et al. FOXM1: A small fox that makes more tracks for cancer progression and metastasis. Semin Cancer Biol. 2023 Jul
2. Zhang Z et al. FOXM1: Functional Roles of FOXM1 in Non-Malignant Diseases. Biomolecules. 2023 May
Subcellular Location
Nucleus.
Synonyms
FKHL16 antibody
Forkhead box M1 antibody
Forkhead box protein M1 antibody
forkhead like 16 antibody
Forkhead-related protein FKHL16 antibody
FOX M1 antibody
Foxm1 antibody
FOXM1_HUMAN antibody
FOXM1B antibody
Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
ExpandImages
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This data was developed using HA723197, the same antibody clone in a different buffer formulation.
Western blot analysis of FOXM1 on different lysates with Rabbit anti-FOXM1 antibody (HA723197) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: LO2 cell lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 84 kDa
Observed band size: 110 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723197) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723197, the same antibody clone in a different buffer formulation.
FOXM1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723197 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723197 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA723197 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723197 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 4 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"