SV2A Recombinant Rabbit Monoclonal Antibody [PSH10-69] - BSA and Azide free
Catalog# HA751371
SV2A Recombinant Rabbit Monoclonal Antibody [PSH10-69] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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IP
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
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unconjugated
Overview
Product Name
SV2A Recombinant Rabbit Monoclonal Antibody [PSH10-69] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within N terminal Human SV2A.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IP
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
Human cerebellum tissue, mouse cerebellum tissue, mouse pancreas tissue, rat cerebellum tissue, rat pancreas tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-69
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Synaptic vesicle glycoprotein 2A (SV2A) is a transmembrane protein belonging to a family of keratan sulfate proteoglycans, located on the synaptic vesicles of mammalian neuronal and endocrine cells. It's encoded by the SV2A gene. SV2A is the most widely expressed isoform of the SV2 family (which also includes the SV2B and SV2C proteins) found in all brain regions. The role of the SV2 proteins is not well understood, however they are thought to be involved in regulating vesicular processes. The SV2A protein is a target of the anti-epileptic drugs (anticonvulsants) levetiracetam and brivaracetam but it is not clear how these drug affect SV2A activity.
Background References
1. Bavarsad MS et al. SV2A PET imaging in human neurodegenerative diseases. Front Aging Neurosci. 2024 Apr
2. Wu PP et al. Development of SV2A Ligands for Epilepsy Treatment: A Review of Levetiracetam, Brivaracetam, and Padsevonil. Neurosci Bull. 2024 May
Subcellular Location
Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
Synonyms
KIAA0736 antibody
OTTHUMP00000014065 antibody
SV2 antibody
Sv2a antibody
SV2A_HUMAN antibody
Synaptic vesicle glycoprotein 2 antibody
Synaptic vesicle glycoprotein 2A antibody
Synaptic vesicle protein 2a antibody
Images
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This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SV2A antibody (HA723233) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723233) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-SV2A antibody (HA723233) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723233) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-SV2A antibody (HA723233) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723233) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SV2A antibody (HA723233) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723233) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-SV2A antibody (HA723233) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723233) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
Western blot analysis of SV2A on different lysates with Rabbit anti-SV2A antibody (HA723233) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: NIH/3T3 cell lysate (negative)
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723233) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723233, the same antibody clone in a different buffer formulation.
SV2A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA723233 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723233 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: mouse brain tissue lysate (input)
Lane 2: HA723233 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA723233 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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