MICA + MICB Recombinant Rabbit Monoclonal Antibody [PSH10-88] - BSA and Azide free
Catalog# HA751380
MICA + MICB Recombinant Rabbit Monoclonal Antibody [PSH10-88] - BSA and Azide free
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WB
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FC
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IP
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Human
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HA723255
含抗保成分
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unconjugated
Overview
Product Name
MICA + MICB Recombinant Rabbit Monoclonal Antibody [PSH10-88] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MICB aa 1-309.
Species Reactivity
Human
Validated Applications
WB, FC, IP
Molecular Weight
Predicted band size: 43 kDa
Positive Control
HeLa cell lysate, MCF7 cell lysate, NIH:OVCAR-3 cell lysate, U-2 OS cell lysate, HUVEC cell lysate, HeLa.
Conjugation
unconjugated
Clone Number
PSH10-88
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
MHC class I polypeptide–related sequence A (MICA) is a highly polymorphic cell surface glycoprotein encoded by the MICA gene located within MHC locus. MICA is related to MHC class I and it has similar domain structure, however, it is not associated with β2-microglobulin nor binds peptides as conventional MHC class I molecules do. MICA rather functions as a stress-induced ligand (as a danger signal) for integral membrane protein receptor NKG2D ("natural-killer group 2, member D"). MICA is broadly recognized by NK cells, γδ T cells, and CD8+ αβ T cells which carry NKG2D receptor on their cell surface and which are activated via this interaction. MHC class I polypeptide-related sequence B (MICB) is a protein that is encoded by the MICB gene located within MHC locus. MICB is related to MHC class I and has similar domain structure, which is made up of external α1α2α3 domain, transmembrane segment and C-terminal cytoplasmic tail. MICB is a stress-induced ligand for NKG2D receptor. The heat shock stress pathway is involved in the regulation of MICB expression as transcription of MICB is regulated by promoter heat shock element.
Background References
1. Mao D et al. Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer. Cancer Lett. 2023 Sep
2. Aguilar OA et al. MICB Genomic Variant Is Associated with NKG2D-mediated Acute Lung Injury and Death. Am J Respir Crit Care Med. 2024 Jan
Subcellular Location
Cell membrane, Cytoplasm; Cell membrane.
Synonyms
HLA class I antigen antibody
FLJ36918 antibody
FLJ60820 antibody
MGC111087 antibody
MGC21250 antibody
MHC class I chain related gene A protein antibody
MHC class I chain related protein A antibody
MHC class I chain related protein A HLA B HLA C antibody
MHC class I polypeptide related sequence A antibody
MHC class I polypeptide-related sequence A antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA751380) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: MCF7 cell lysate (25 µg/Lane)
Lane 3: THP-1 cell lysate (negative) (25 µg/Lane)
Lane 4: NIH:OVCAR-3 cell lysate (25 µg/Lane)
Lane 5: PC-3 cell lysate (low expression) (25 µg/Lane)
Lane 6: U-2 OS cell lysate (25 µg/Lane)
Lane 7: HUVEC cell lysate (25 µg/Lane)
Predicted band size: 43 kDa
Observed band size: 60 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751380) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA751380) at 1/2,000 dilution.
Lane 1: Human MICA recombinant protein, 20ng/Lane
Lane 2: Human MICB recombinant protein, 20ng/Lane
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751380) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA + MICB.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751380, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
MICA + MICB was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751380 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751380 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA751380 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA751380 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 7 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"