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ROR alpha / RORA Recombinant Rabbit Monoclonal Antibody [PSH11-00] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751390

ROR alpha / RORA Recombinant Rabbit Monoclonal Antibody [PSH11-00] - BSA and Azide free

Application

  • IHC-P

  • WB

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ROR alpha / RORA Recombinant Rabbit Monoclonal Antibody [PSH11-00] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse RORA aa aa 101-523.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-P, WB

Molecular Weight

Predicted band size: 59 kDa

Positive Control

Mouse cerebellum tissue, Rat cerebellum tissue, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH11-00

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000

  • WB

  • 1:2,000

Target

Function

RAR-related orphan receptor alpha (RORα), also known as NR1F1 (nuclear receptor subfamily 1, group F, member 1) is a nuclear receptor that in humans is encoded by the RORA gene. RORα participates in the transcriptional regulation of some genes involved in circadian rhythm. In mice, RORα is essential for development of cerebellum through direct regulation of genes expressed in Purkinje cells. It also plays an essential role in the development of type 2 innate lymphoid cells (ILC2) and mutant animals are ILC2 deficient. In addition, although present in normal numbers, the ILC3 and Th17 cells from RORα deficient mice are defective for cytokine production.

Background References

1. Liu D et al. The Circadian Clock Component RORA Increases Immunosurveillance in Melanoma by Inhibiting PD-L1 Expression. Cancer Res. 2024 Jul

2. Li M et al. The single-cell transcriptomic atlas and RORA-mediated 3D epigenomic remodeling in driving corneal epithelial differentiation. Nat Commun. 2024 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P51448 Mouse

Entrez Gene: 300807 Rat

Synonyms

fhl2a antibody

NR1F1 antibody

Nuclear receptor ROR alpha antibody

Nuclear receptor ROR-alpha antibody

Nuclear receptor RZR-alpha antibody

Nuclear receptor subfamily 1 group F member 1 antibody

RAR related orphan receptor A antibody

RAR related orphan receptor alpha antibody

RAR-related orphan receptor A antibody

Retinoid-related orphan receptor-alpha antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-ROR alpha / RORA antibody (<a href="/products/HA751390" style="font-weight: bold;text-decoration: underline;">HA751390</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751390" style="font-weight: bold;text-decoration: underline;">HA751390</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-ROR alpha / RORA antibody (HA751390) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751390) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-ROR alpha / RORA antibody (<a href="/products/HA751390" style="font-weight: bold;text-decoration: underline;">HA751390</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751390" style="font-weight: bold;text-decoration: underline;">HA751390</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-ROR alpha / RORA antibody (HA751390) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751390) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ROR alpha / RORA on different lysates with Rabbit anti-ROR alpha / RORA antibody (<a href="/products/HA751390" style="font-weight: bold;text-decoration: underline;">HA751390</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 2: Mouse cerebellum tissue lysate (40 µg/Lane)<br />Lane 3: Mouse spleen tissue lysate (negative) (40 µg/Lane)<br />Lane 4: Rat cerebellum tissue lysate (40 µg/Lane)<br />Lane 5: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 59 kDa<br />Observed band size: 56/59 kDa<br />Exposure time: 3 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751390" style="font-weight: bold;text-decoration: underline;">HA751390</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ROR alpha / RORA on different lysates with Rabbit anti-ROR alpha / RORA antibody (HA751390) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (40 µg/Lane)
    Lane 2: Mouse cerebellum tissue lysate (40 µg/Lane)
    Lane 3: Mouse spleen tissue lysate (negative) (40 µg/Lane)
    Lane 4: Rat cerebellum tissue lysate (40 µg/Lane)
    Lane 5: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 59 kDa
    Observed band size: 56/59 kDa
    Exposure time: 3 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751390) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"