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FAM38B / PIEZO2 Recombinant Rabbit Monoclonal Antibody [PSH11-50] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751412

FAM38B / PIEZO2 Recombinant Rabbit Monoclonal Antibody [PSH11-50] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

FAM38B / PIEZO2 Recombinant Rabbit Monoclonal Antibody [PSH11-50] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse PIEZO2 aa 2,497-2,731.

Species Reactivity

Mouse

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 326 kDa

Positive Control

293T transfected with mPiezo2 cell lysate.

Conjugation

unconjugated

Clone Number

PSH11-50

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:200

Target

Function

Piezo-type mechanosensitive ion channel component 2 is a protein that in humans is encoded by the PIEZO2 gene. It has a homotrimeric structure, with three blades curving into a nano-dome, with a diameter of 28 nanometers. Piezos are large transmembrane proteins conserved among various species, all having between 24 and 36 predicted transmembrane domains. 'Piezo' comes from the Greek 'piesi,' meaning 'pressure.' The PIEZO2 protein has a role in rapidly adapting mechanically activated (MA) currents in somatosensory neurons. Its structure is resolved via a mouse version in 2019, showing the predicted homotrimeric propeller. PIEZO2 is typically found in cell types that respond to physical touch, such as Merkel cells, and is thought to regulate light touch response.

Background References

1. Szczot M et al. The Form and Function of PIEZO2. Annu Rev Biochem. 2021 Jun

2. Servin-Vences MR et al. PIEZO2 in somatosensory neurons controls gastrointestinal transit. Cell. 2023 Aug

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q8CD54 Mouse

Synonyms

Piezo-type mechanosensitive ion channel component 2

Protein FAM38B

Piezo2

Fam38b

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of FAM38B / PIEZO2 on different lysates with Rabbit anti-FAM38B / PIEZO2 antibody (<a href="/products/HA751412" style="font-weight: bold;text-decoration: underline;">HA751412</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T transfected with mPiezo1 cell lysate (negative)<br />Lane 2: 293T transfected with mPiezo2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 326 kDa<br />Observed band size: 326 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751412" style="font-weight: bold;text-decoration: underline;">HA751412</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of FAM38B / PIEZO2 on different lysates with Rabbit anti-FAM38B / PIEZO2 antibody (HA751412) at 1/2,000 dilution.

    Lane 1: 293T transfected with mPiezo1 cell lysate (negative)
    Lane 2: 293T transfected with mPiezo2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 326 kDa
    Observed band size: 326 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751412) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of 293T cells labeling FAM38B / PIEZO2 with Rabbit anti-FAM38B / PIEZO2 antibody (<a href="/products/HA751412" style="font-weight: bold;text-decoration: underline;">HA751412</a>) at 1/200 dilution.<br /><br />293T cells, transfected with GFP-tagged mouse Piezo1 (top, negative) / tag-free mouse Piezo2 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38B / PIEZO2 antibody (<a href="/products/HA751412" style="font-weight: bold;text-decoration: underline;">HA751412</a>) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of 293T cells labeling FAM38B / PIEZO2 with Rabbit anti-FAM38B / PIEZO2 antibody (HA751412) at 1/200 dilution.

    293T cells, transfected with GFP-tagged mouse Piezo1 (top, negative) / tag-free mouse Piezo2 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38B / PIEZO2 antibody (HA751412) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"