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IGF1 Receptor beta Recombinant Rabbit Monoclonal Antibody [PSH11-72] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751427

IGF1 Receptor beta Recombinant Rabbit Monoclonal Antibody [PSH11-72] - BSA and Azide free

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

IGF1 Receptor beta Recombinant Rabbit Monoclonal Antibody [PSH11-72] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human IGF1 Receptor aa 701-935.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 155 kDa

Positive Control

HEK-293 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, A431 cell lysate, HepG2 cell lysate, A549 cell lysate, HeLa cell lysate, HUVEC cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NIH/3T3.

Conjugation

unconjugated

Clone Number

PSH11-72

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

The insulin-like growth factor 1 (IGF-1) receptor is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called insulin-like growth factor 1 (IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF-1 plays an important role in growth and continues to have anabolic effects in adults – meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Mice lacking the IGF-1 receptor die late in development, and show a dramatic reduction in body mass. This testifies to the strong growth-promoting effect of this receptor.

Background References

1. Krieger CC et al. TSH/IGF1 receptor crosstalk: Mechanism and clinical implications. Pharmacol Ther. 2020 May

2. Shin JW et al. Grabody B, an IGF1 receptor-based shuttle, mediates efficient delivery of biologics across the blood-brain barrier. Cell Rep Methods. 2022 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P08069 Human

SwissProt: Q60751 Mouse

SwissProt: P24062 Rat

Synonyms

CD221 antibody

CD221 antigen antibody

IGF 1 receptor antibody

IGF 1R antibody

IGF I receptor antibody

IGF-I receptor antibody

Igf1r antibody

IGF1R_HUMAN antibody

IGFIR antibody

IGFIRC antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of IGF1 Receptor beta on different lysates with Rabbit anti-IGF1 Receptor beta antibody (<a href="/products/HA751427" style="font-weight: bold;text-decoration: underline;">HA751427</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: MDA-MB-231 cell lysate (low expression) (20 µg/Lane)<br />Lane 4: A431 cell lysate (20 µg/Lane)<br />Lane 5: HepG2 cell lysate (20 µg/Lane)<br />Lane 6: A549 cell lysate (20 µg/Lane)<br />Lane 7: HeLa cell lysate (20 µg/Lane)<br />Lane 8: HUVEC cell lysate (20 µg/Lane)<br />Lane 9: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 10: C2C12 cell lysate (20 µg/Lane)<br />Lane 11: C6 cell lysate (20 µg/Lane)<br />Lane 12: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 13: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 155 kDa<br />Observed band size: 95 kDa<br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751427" style="font-weight: bold;text-decoration: underline;">HA751427</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of IGF1 Receptor beta on different lysates with Rabbit anti-IGF1 Receptor beta antibody (HA751427) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: MDA-MB-231 cell lysate (low expression) (20 µg/Lane)
    Lane 4: A431 cell lysate (20 µg/Lane)
    Lane 5: HepG2 cell lysate (20 µg/Lane)
    Lane 6: A549 cell lysate (20 µg/Lane)
    Lane 7: HeLa cell lysate (20 µg/Lane)
    Lane 8: HUVEC cell lysate (20 µg/Lane)
    Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 10: C2C12 cell lysate (20 µg/Lane)
    Lane 11: C6 cell lysate (20 µg/Lane)
    Lane 12: Mouse brain tissue lysate (40 µg/Lane)
    Lane 13: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 155 kDa
    Observed band size: 95 kDa
    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751427) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of NIH/3T3 cells labeling IGF1 Receptor beta with Rabbit anti-IGF1 Receptor beta antibody (<a href="/products/HA751427" style="font-weight: bold;text-decoration: underline;">HA751427</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGF1 Receptor beta antibody (<a href="/products/HA751427" style="font-weight: bold;text-decoration: underline;">HA751427</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NIH/3T3 cells labeling IGF1 Receptor beta with Rabbit anti-IGF1 Receptor beta antibody (HA751427) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGF1 Receptor beta antibody (HA751427) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"