AFP Recombinant Rabbit Monoclonal Antibody [PSH11-75] - BSA and Azide free
Catalog# HA751430
AFP Recombinant Rabbit Monoclonal Antibody [PSH11-75] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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HA723371
含抗保成分
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unconjugated
Overview
Product Name
AFP Recombinant Rabbit Monoclonal Antibody [PSH11-75] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human AFP aa 1-609.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 69 kDa
Positive Control
HepG2 cell lysate, Mouse placenta tissue lysate, human placenta tissue, human poorly differentiated hepatocellular carcinoma tissue, mouse embryo liver tissue, rat embryo liver tissue, HepG2.
Conjugation
unconjugated
Clone Number
PSH11-75
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:250
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IHC-P
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1:20,000
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IF-Tissue
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1:1,000
Target
Function
Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q13.3). Maternal AFP serum level is used to screen for Down syndrome, neural tube defects, and other chromosomal abnormalities. AFP is a major plasma protein produced by the yolk sac and the fetal liver during fetal development. It is thought to be the fetal analog of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms.
Background References
1. Yeo YH et al. Alpha-fetoprotein: Past, present, and future. Hepatol Commun. 2024 Apr
2. Hu X et al. The Landscape Of Alpha Fetoprotein In Hepatocellular Carcinoma: Where Are We? Int J Biol Sci. 2022 Jan
Subcellular Location
Secreted.
Synonyms
Afp antibody
AFPD antibody
Alpha fetoglobulin antibody
Alpha fetoprotein antibody
Alpha fetoprotein precursor antibody
Alpha-1-fetoprotein antibody
Alpha-fetoglobulin antibody
Alpha-fetoprotein antibody
alpha-fetoprotein, Hereditary persistence of, included antibody
FETA antibody
ExpandImages
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Western blot analysis of AFP on different lysates with Rabbit anti-AFP antibody (HA751430) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Mouse placenta tissue lysate (40 µg/Lane)
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751430) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-AFP antibody (HA751430) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751430) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human poorly differentiated hepatocellular carcinoma tissue with Rabbit anti-AFP antibody (HA751430) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751430) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human well-differentiated hepatocellular carcinoma tissue (negative) with Rabbit anti-AFP antibody (HA751430) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751430) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse embryo liver tissue with Rabbit anti-AFP antibody (HA751430) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751430) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat embryo liver tissue with Rabbit anti-AFP antibody (HA751430) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751430) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HepG2 cells labeling AFP with Rabbit anti-AFP antibody (HA751430) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AFP antibody (HA751430) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Application: IF-Tissue
Species: Human
Site: poorly differentiated hepatocellular carcinoma
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
Application: IF-Tissue
Species: Human
Site: liver (negative)
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"