LARP1 Recombinant Rabbit Monoclonal Antibody [PSH12-09] - BSA and Azide free
Catalog# HA751438
LARP1 Recombinant Rabbit Monoclonal Antibody [PSH12-09] - BSA and Azide free
-
WB
-
IF-Cell
-
Human
-
Green monkey
-
HA723425
含抗保成分
-
unconjugated
Overview
Product Name
LARP1 Recombinant Rabbit Monoclonal Antibody [PSH12-09] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LARP1 aa 181-330.
Species Reactivity
Human, Green monkey
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 124 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, 786-0 cell lysate, COS-1 cell lysate, HeLa, COS-1.
Conjugation
unconjugated
Clone Number
PSH12-09
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IF-Cell
-
1:50-1:100
Target
Function
La-related protein 1 (LARP1) is a 150 kDa protein that in humans is encoded by the LARP1 gene. LARP1 is a novel target of the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway, a circuitry often hyperactivated in cancer which regulates cell growth and proliferation primarily through the regulation of protein synthesis. LARP1 is the largest of a 7-member family of LARP proteins (others are: LARP1B, LARP3 (aka genuine La or SSB), LARP4A, LARP4B, LARP6 and LARP7). All LARP proteins, including human LARPs, contain 2 conserved regions. The first conserved region shares homology with La proteins (called the La motif, see SSB) whereas the second conserved region (called the LA- motif) is restricted to LARP proteins. LARP1 and 1B also contain a conserved "DM15 region" within their C-terminus. This region is unique and has been shown to be required for RNA-binding. Mouse Larp1 is expressed in dorsal root ganglia and spinal cord, as well as in developing organs characterized by epithelial-mesenchymal interactions. Human LARP1 is present at low levels in normal, non-embryonic cells but is highly expressed in epithelial cancers (such as ovarian, colorectal, prostate, non-small cell lung, hepatocellular and cervical cancers). Some studies have shown that high levels of LARP1 protein correlate with worse prognosis in cancer patients.
Background References
1. Berman AJ et al. Controversies around the function of LARP1. RNA Biol. 2021 Feb
2. Mattijssen S et al. LARP1 and LARP4: up close with PABP for mRNA 3\' poly(A) protection and stabilization. RNA Biol. 2021 Feb
Subcellular Location
Cytoplasm, Cytoplasmic granule.
UNIPROT
Synonyms
1810024J12Rik antibody
3110040D16Rik antibody
KIAA0731 antibody
La related protein antibody
La ribonucleoprotein domain family, member 1 antibody
LARP antibody
MGC19556 antibody
mKIAA0731 antibody
rCG_32791 antibody
RGD1306683 antibody
ExpandImages
-
Western blot analysis of LARP1 on different lysates with Rabbit anti-LARP1 antibody (HA751438) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 786-0 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 150 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751438) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling LARP1 with Rabbit anti-LARP1 antibody (HA751438) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LARP1 antibody (HA751438) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of COS-1 cells labeling LARP1 with Rabbit anti-LARP1 antibody (HA751438) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LARP1 antibody (HA751438) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"