BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52] - BSA and Azide free
Catalog# HA751471
BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52] - BSA and Azide free
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WB
-
IP
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ChIP
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Human
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HA723541
含抗保成分
-
unconjugated
Overview
Product Name
BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human BATF3 aa 1-127.
Species Reactivity
Human
Validated Applications
WB, IP, ChIP
Molecular Weight
Predicted band size: 14 kDa
Positive Control
U-937 cell lysate, HDLM2 cell lysate.
Conjugation
unconjugated
Clone Number
PSH13-52
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
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IP
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1-2μg/sample
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ChIP
-
Use 0.5~2 μg for 25 μg of chromatin.
Target
Function
AP-1 family transcription factor that controls the differentiation of CD8+ thymic conventional dendritic cells in the immune system. Required for development of CD8-alpha+ classical dendritic cells (cDCs) and related CD103+ dendritic cells that cross-present antigens to CD8 T-cells and produce interleukin-12 (IL12) in response to pathogens (By similarity). Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes.
Background References
1. Jain N et al. TET2 guards against unchecked BATF3-induced CAR T cell expansion. Nature. 2023 Mar
2. Ziblat A et al. Batf3(+) DCs and the 4-1BB/4-1BBL axis are required at the effector phase in the tumor microenvironment for PD-1/PD-L1 blockade efficacy. Cell Rep. 2024 May
Subcellular Location
Nucleus.
UNIPROT
Synonyms
21 kD small nuclear factor isolated from T cells antibody
21 kDa small nuclear factor isolated from T-cells antibody
ATF like 3 antibody
B-ATF-3 antibody
basic leucine zipper transcription factor, ATF-like 3 antibody
BATF3 antibody
FLJ36352 antibody
FLJ37535 antibody
hCG 39202 antibody
JDP1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of BATF3 on different lysates with Rabbit anti-BATF3 antibody (HA751471) at 1/5,000 dilution.
Lane 1: U-937 cell lysate
Lane 2: Ramos cell lysate (negative)
Lane 3: HDLM2 cell lysate
Lane 4: HepG2 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 18 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751471) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
BATF3 was immunoprecipitated from 0.2 mg U-937 cell lysate with HA751471 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751471 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: U-937 cell lysate (input)
Lane 2: HA751471 IP in U-937 cell lysate
Lane 3: Rabbit IgG instead of HA751471 in U-937 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 minutes; ECL: K1801 -
Chromatin immunoprecipitations were performed with cross-linked chromatin from U-937 cells with BATF3 (HA751471) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"