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BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751471

BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52] - BSA and Azide free

Application

  • WB

  • IP

  • ChIP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

BATF3 Recombinant Rabbit Monoclonal Antibody [PSH13-52] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human BATF3 aa 1-127.

Species Reactivity

Human

Validated Applications

WB, IP, ChIP

Target Molecular Weight

Predicted band size: 14 kDa

Positive Control

U-937 cell lysate, HDLM2 cell lysate.

Conjugation

unconjugated

Clone Number

PSH13-52

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

AP-1 family transcription factor that controls the differentiation of CD8+ thymic conventional dendritic cells in the immune system. Required for development of CD8-alpha+ classical dendritic cells (cDCs) and related CD103+ dendritic cells that cross-present antigens to CD8 T-cells and produce interleukin-12 (IL12) in response to pathogens (By similarity). Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes.

Background References

1. Jain N et al. TET2 guards against unchecked BATF3-induced CAR T cell expansion. Nature. 2023 Mar

2. Ziblat A et al. Batf3(+) DCs and the 4-1BB/4-1BBL axis are required at the effector phase in the tumor microenvironment for PD-1/PD-L1 blockade efficacy. Cell Rep. 2024 May

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9NR55 Human

Synonyms

21 kD small nuclear factor isolated from T cells antibody

21 kDa small nuclear factor isolated from T-cells antibody

ATF like 3 antibody

B-ATF-3 antibody

basic leucine zipper transcription factor, ATF-like 3 antibody

BATF3 antibody

FLJ36352 antibody

FLJ37535 antibody

hCG 39202 antibody

JDP1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of BATF3 on different lysates with Rabbit anti-BATF3 antibody (<a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a>) at 1/5,000 dilution.<br /><br />Lane 1: U-937 cell lysate<br />Lane 2: Ramos cell lysate (negative)<br />Lane 3: HDLM2 cell lysate<br />Lane 4: HepG2 cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 18 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    This data was developed using HA723541, the same antibody clone in a different buffer formulation.
    Western blot analysis of BATF3 on different lysates with Rabbit anti-BATF3 antibody (HA723541) at 1/5,000 dilution.

    Lane 1: U-937 cell lysate
    Lane 2: Ramos cell lysate (negative)
    Lane 3: HDLM2 cell lysate
    Lane 4: HepG2 cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 18 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723541) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a>, the same antibody clone in a different buffer formulation.<br />BATF3 was immunoprecipitated from 0.2 mg U-937 cell lysate with <a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: U-937 cell lysate (input)<br />Lane 2: <a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a> IP in U-937 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a> in U-937 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    This data was developed using HA723541, the same antibody clone in a different buffer formulation.
    BATF3 was immunoprecipitated from 0.2 mg U-937 cell lysate with HA723541 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723541 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: U-937 cell lysate (input)
    Lane 2: HA723541 IP in U-937 cell lysate
    Lane 3: Rabbit IgG instead of HA723541 in U-937 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 3 minutes; ECL: K1801

  • This data was developed using <a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a>, the same antibody clone in a different buffer formulation.<br />Chromatin immunoprecipitations were performed with cross-linked chromatin from U-937 cells with BATF3 (<a href="/products/HA723541" style="font-weight: bold;text-decoration: underline;">HA723541</a>) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    This data was developed using HA723541, the same antibody clone in a different buffer formulation.
    Chromatin immunoprecipitations were performed with cross-linked chromatin from U-937 cells with BATF3 (HA723541) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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