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QKI-5 Recombinant Antibody [PSH10-22] - Rabbit IgG (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751496

QKI-5 Recombinant Antibody [PSH10-22] - Rabbit IgG (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

QKI-5 Recombinant Antibody [PSH10-22] - Rabbit IgG (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within human QKI-5 aa 301-341 / 341.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB

Target Molecular Weight

Predicted band size: 38 kDa

Positive Control

Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-22

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Quaking homolog, KH domain RNA binding (mouse), also known as QKI, is a protein which in humans is encoded by the QKI gene. QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1. Two more new members are KHDRBS3 and KHDRBS2. The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.

Background References

1. Zhao Z et al. QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolism. Cell. 2023 Jul

2. Suh YY et al. MYB/MYBL1::QKI fusion-positive diffuse glioma. J Neuropathol Exp Neurol. 2023 Feb

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q96PU8-1 Human

SwissProt: Q9QYS9 Mouse

SwissProt: Q91XU1 Rat

Synonyms

DKFZp586I0923 antibody

HKQ antibody

Homolog of mouse quaking QKI KH domain RNA binding protein antibody

Hqk antibody

HQK1 antibody

HqkI antibody

OTTHUMP00000017581 antibody

OTTHUMP00000017582 antibody

OTTHUMP00000017583 antibody

Protein quaking antibody

Expand

Images

  • This data was developed using <a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    This data was developed using HA723587, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-QKI-5 antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA723587, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-QKI-5 antibody (HA723587) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723587) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-QKI-5 antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA723587, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-QKI-5 antibody (HA723587) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723587) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-QKI-5 antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA723587, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-QKI-5 antibody (HA723587) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723587) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-QKI-5 antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA723587, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-QKI-5 antibody (HA723587) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723587) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of QKI-5 on different lysates with Rabbit anti-QKI-5 antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723587" style="font-weight: bold;text-decoration: underline;">HA723587</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA723587, the same antibody clone in a different buffer formulation.
    Western blot analysis of QKI-5 on different lysates with Rabbit anti-QKI-5 antibody (HA723587) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723587) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

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