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Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-66] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751527

Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-66] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-66] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Granzyme B aa 1-247.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 28 kDa

Positive Control

NK-92 cell lysate, CTLL-2 cell lysate, human NK/T-cell lymphoma tissue, human spleen tissue.

Conjugation

unconjugated

Clone Number

PSH14-66

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100,000

  • IHC-P

  • 1:500-1:5,000

Target

Function

Granzyme B (GrB) is one of the serine protease granzymes most commonly found in the granules of natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells. Granzyme B has also been found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. The secondary functions of granzyme B are also numerous. Granzyme B has shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodelling. Elevated levels of granzyme B are also implicated in a number of autoimmune diseases, several skin diseases, and type 1 diabetes.

Background References

1. Gleave A et al. Granzyme B in Autoimmune Skin Disease. Biomolecules. 2023 Feb

2. Jung K et al. Granzyme B in epithelial barrier dysfunction and related skin diseases. Am J Physiol Cell Physiol. 2022 Jul

Subcellular Location

Secreted, Cytolytic granule.

UNIPROT

SwissProt: P10144 Human

SwissProt: P04187 Mouse

Synonyms

C11 antibody

Cathepsin G like 1 antibody

Cathepsin G-like 1 antibody

CCPI antibody

CGL 1 antibody

CGL1 antibody

CSP B antibody

CSPB antibody

CTLA 1 antibody

CTLA-1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Granzyme B on different lysates with Rabbit anti-Granzyme B antibody (<a href="/products/HA751527" style="font-weight: bold;text-decoration: underline;">HA751527</a>) at 1/100,000 dilution.<br /><br />Lane 1: NK-92 cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (negative) (20 µg/Lane)<br />Lane 3: CTLL-2 cell lysate (20 µg/Lane)<br />Lane 4: C2C12 cell lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 28 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751527" style="font-weight: bold;text-decoration: underline;">HA751527</a>) at 1/100,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Granzyme B on different lysates with Rabbit anti-Granzyme B antibody (HA751527) at 1/100,000 dilution.

    Lane 1: NK-92 cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (negative) (20 µg/Lane)
    Lane 3: CTLL-2 cell lysate (20 µg/Lane)
    Lane 4: C2C12 cell lysate (negative) (20 µg/Lane)

    Predicted band size: 28 kDa
    Observed band size: 30 kDa

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751527) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue with Rabbit anti-Granzyme B antibody (<a href="/products/HA751527" style="font-weight: bold;text-decoration: underline;">HA751527</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751527" style="font-weight: bold;text-decoration: underline;">HA751527</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue with Rabbit anti-Granzyme B antibody (HA751527) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751527) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Granzyme B antibody (<a href="/products/HA751527" style="font-weight: bold;text-decoration: underline;">HA751527</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751527" style="font-weight: bold;text-decoration: underline;">HA751527</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Granzyme B antibody (HA751527) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751527) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"