Hamartin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH14-67] (HA751528)
Catalog# HA751528
Rabbit Monoclonal to Hamartin
Tested for
-
WB
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Hamartin on different lysates with Rabbit anti-Hamartin antibody (HA751528) at 1/20,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: COS-1 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: MEF cell lysate
Lane 6: C2C12 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate
Lane 9: Mouse brain tissue lysate
Lane 10: Mouse heart tissue lysate
Lane 11: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 130 kDa
Observed band size: 140 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751528) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: COS-1 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: MEF cell lysate
Lane 6: C2C12 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate
Lane 9: Mouse brain tissue lysate
Lane 10: Mouse heart tissue lysate
Lane 11: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 130 kDa
Observed band size: 140 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751528) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.