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Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody [PSH14-70] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751530

Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody [PSH14-70] - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody [PSH14-70] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Homeobox protein cut-like 1 aa 1,000-1,200.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P

Molecular Weight

Predicted band size: 166 kDa

Positive Control

Mouse cerebrum tissue, rat cerebrum tissue.

Conjugation

unconjugated

Clone Number

PSH14-70

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200-1:500

  • IHC-P

  • 1:500-1:1,000

Target

Function

Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. Also involved in the control of synaptogenesis (Probable). In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.

Background References

1. Liu N et al. CUX1, A Controversial Player in Tumor Development. Front Oncol. 2020 May

2. Jotte MRM et al. The significance of CUX1 and chromosome 7 in myeloid malignancies. Curr Opin Hematol. 2022 Mar

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P53564 Mouse

SwissProt: P53565 Rat

Synonyms

CCAAT displacement protein antibody

CDP antibody

CDP/Cut antibody

CDP1 antibody

Clox antibody

COY1 antibody

Cut homeobox antibody

Cut homolog antibody

Cut like 1 antibody

Cut like homeobox 1 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: cerebrum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Application: IHC-Fr

    Species: Mouse

    Site: cerebrum

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: cerebrum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: cerebrum

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (<a href="/products/HA751530" style="font-weight: bold;text-decoration: underline;">HA751530</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751530" style="font-weight: bold;text-decoration: underline;">HA751530</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (HA751530) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751530) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (<a href="/products/HA751530" style="font-weight: bold;text-decoration: underline;">HA751530</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751530" style="font-weight: bold;text-decoration: underline;">HA751530</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody (HA751530) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751530) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"