Ly6g Recombinant Rabbit Monoclonal Antibody [PSH14-95] - BSA and Azide free
Catalog# HA751538
Ly6g Recombinant Rabbit Monoclonal Antibody [PSH14-95] - BSA and Azide free
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FC
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IHC-P
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Mouse
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unconjugated
Overview
Product Name
Ly6g Recombinant Rabbit Monoclonal Antibody [PSH14-95] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Ly6g aa 27-105.
Species Reactivity
Mouse
Validated Applications
FC, IHC-P
Target Molecular Weight
Predicted band size: 14 kDa
Positive Control
Mouse bone marrow, C57 mouse blood, mouse spleen tissue, mouse lung tissue.
Conjugation
unconjugated
Clone Number
PSH14-95
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
Background References
1. Ruscitti C et al. Recruited atypical Ly6G(+) macrophages license alveolar regeneration after lung injury. Sci Immunol. 2024 Aug
2. Iliakis CS et al. Never trust a single myeloid marker: Ly6G on repair-promoting lung macrophages. Sci Immunol. 2024 Aug
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Gr-1 antibody
Gr1 antibody
Ly-6G.1 antibody
Ly6G antibody
Lymphocyte antigen 6 complex locus G antibody
Lymphocyte antigen 6G antibody
Images
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This data was developed using HA723696, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of mouse bone marrow cells labeling Ly6g.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723696, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
This data was developed using HA723696, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of C57 mouse blood cells labeling Ly6g.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723696, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
This data was developed using HA723696, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ly6g antibody (HA723696) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723696) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723696, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ly6g antibody (HA723696) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723696) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"