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TAZ / WWTR1 Recombinant Rabbit Monoclonal Antibody [PSH15-57] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751568

TAZ / WWTR1 Recombinant Rabbit Monoclonal Antibody [PSH15-57] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TAZ / WWTR1 Recombinant Rabbit Monoclonal Antibody [PSH15-57] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TAZ / WWTR1 aa 1-400.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 44 kDa

Positive Control

HeLa cell lysate, PANC-1 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, COS-1 cell lysate, F9 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate.

Conjugation

unconjugated

Clone Number

PSH15-57

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

WW domain-containing transcription regulator protein 1 (WWTR1), also known as Transcriptional coactivator with PDZ-binding motif (TAZ), is a protein that in humans is encoded by the WWTR1 gene. WWTR1 acts as a transcriptional coregulator and has no effect on transcription alone. When in complex with transcription factor binding partners, WWTR1 helps promote gene expression in pathways associated with development, cell growth and survival, and inhibiting apoptosis. Aberrant WWTR1 function has been implicated for its role in driving cancers. WWTR1 is often referred to as TAZ due to its initial characterization with the name TAZ. However, WWTR1 (TAZ) is not to be confused with the protein tafazzin, which originally held the official gene symbol TAZ, and is now TAFAZZIN.

Background References

1. Driskill JH et al. WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid hemangioendothelioma. Genes Dev. 2021 Apr

2. Seavey CN et al. WWTR1(TAZ)-CAMTA1 gene fusion is sufficient to dysregulate YAP/TAZ signaling and drive epithelioid hemangioendothelioma tumorigenesis. Genes Dev. 2021 Apr

Subcellular Location

Nucleus, Cytoplasm, Cell membrane, Cell junction, tight junction.

UNIPROT

SwissProt: Q9GZV5 Human

SwissProt: Q9EPK5 Mouse

Entrez Gene: 295062 Rat

Synonyms

DKFZP586I1419 antibody

FLJ27004 antibody

FLJ45718 antibody

OTTHUMP00000215994 antibody

OTTHUMP00000215995 antibody

OTTHUMP00000215996 antibody

OTTHUMP00000216001 antibody

TAZ antibody

Transcriptional co activator with PDZ binding motif antibody

Transcriptional coactivator with PDZ binding motif antibody

Expand

Images

  • Western blot analysis of TAZ / WWTR1 on different lysates with Rabbit anti-TAZ / WWTR1 antibody (<a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: PANC-1 cell lysate<br />Lane 3: SH-SY5Y cell lysate (low expression)<br />Lane 4: SK-MEL-28 cell lysate<br />Lane 5: Raji cell lysate (negative)<br />Lane 6: A375 cell lysate<br />Lane 7: COS-1 cell lysate<br />Lane 8: F9 cell lysate<br />Lane 9: C2C12 cell lysate<br />Lane 10: NIH/3T3 cell lysate<br />Lane 11: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TAZ / WWTR1 on different lysates with Rabbit anti-TAZ / WWTR1 antibody (HA751568) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: PANC-1 cell lysate
    Lane 3: SH-SY5Y cell lysate (low expression)
    Lane 4: SK-MEL-28 cell lysate
    Lane 5: Raji cell lysate (negative)
    Lane 6: A375 cell lysate
    Lane 7: COS-1 cell lysate
    Lane 8: F9 cell lysate
    Lane 9: C2C12 cell lysate
    Lane 10: NIH/3T3 cell lysate
    Lane 11: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 50 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751568) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • TAZ / WWTR1 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: PANC-1 cell lysate (input)<br />Lane 2: <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> IP in PANC-1 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> in PANC-1 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 1 minute 18 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TAZ / WWTR1 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA751568 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751568 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: PANC-1 cell lysate (input)
    Lane 2: HA751568 IP in PANC-1 cell lysate
    Lane 3: Rabbit IgG instead of HA751568 in PANC-1 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 1 minute 18 seconds; ECL: K1801

  • TAZ / WWTR1 was immunoprecipitated from 0.2 mg C2C12 cell lysate with <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: C2C12 cell lysate (input)<br />Lane 2: <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> IP in C2C12 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751568" style="font-weight: bold;text-decoration: underline;">HA751568</a> in C2C12 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 10 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TAZ / WWTR1 was immunoprecipitated from 0.2 mg C2C12 cell lysate with HA751568 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751568 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: C2C12 cell lysate (input)
    Lane 2: HA751568 IP in C2C12 cell lysate
    Lane 3: Rabbit IgG instead of HA751568 in C2C12 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 10 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"