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SIRT5 Recombinant Rabbit Monoclonal Antibody [PSH15-71] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751578

SIRT5 Recombinant Rabbit Monoclonal Antibody [PSH15-71] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SIRT5 Recombinant Rabbit Monoclonal Antibody [PSH15-71] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 34 kDa

Positive Control

293T cell lysate, Hepa-1-6 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Rat liver tissue lysate, human liver tissue.

Conjugation

unconjugated

Clone Number

PSH15-71

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

Target

Function

SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.

Background References

1. Teng P et al. SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration. Cell Death Differ. 2024 Jan

2. Wu M et al. Sirt5 improves cardiomyocytes fatty acid metabolism and ameliorates cardiac lipotoxicity in diabetic cardiomyopathy via CPT2 de-succinylation. Redox Biol. 2024 Jul

Subcellular Location

Mitochondrion matrix, Mitochondrion intermembrane space, Cytoplasm, cytosol, Nucleus.

UNIPROT

SwissProt: Q9NXA8 Human

SwissProt: Q8K2C6 Mouse

SwissProt: Q68FX9 Rat

Synonyms

NAD dependent deacetylase sirtuin 5 antibody

NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody

NAD dependent protein deacylase sirtuin 5 mitochondrial antibody

NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody

Regulatory protein SIR2 homolog 5 antibody

Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody

Sir2 like 5 antibody

SIR2-like protein 5 antibody

SIR2L5 antibody

SIR5_HUMAN antibody

Expand

Images

  • Western blot analysis of SIRT5 on different lysates with Rabbit anti-SIRT5 antibody (<a href="/products/HA751578" style="font-weight: bold;text-decoration: underline;">HA751578</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate (20 µg/Lane)<br />Lane 2: Hepa-1-6 cell lysate (20 µg/Lane)<br />Lane 3: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 34 kDa<br />Observed band size: 34 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751578" style="font-weight: bold;text-decoration: underline;">HA751578</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SIRT5 on different lysates with Rabbit anti-SIRT5 antibody (HA751578) at 1/2,000 dilution.

    Lane 1: 293T cell lysate (20 µg/Lane)
    Lane 2: Hepa-1-6 cell lysate (20 µg/Lane)
    Lane 3: RAW264.7 cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (20 µg/Lane)

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751578) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SIRT5 antibody (<a href="/products/HA751578" style="font-weight: bold;text-decoration: underline;">HA751578</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751578" style="font-weight: bold;text-decoration: underline;">HA751578</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SIRT5 antibody (HA751578) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"