TAP2 Recombinant Rabbit Monoclonal Antibody [PSH16-55] - BSA and Azide free
Catalog# HA751604
TAP2 Recombinant Rabbit Monoclonal Antibody [PSH16-55] - BSA and Azide free
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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HA723859
含抗保成分
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unconjugated
Overview
Product Name
TAP2 Recombinant Rabbit Monoclonal Antibody [PSH16-55] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TAP2 aa 420-686.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 76 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, MoLT-4 cell lysate, SW480 cell lysate, HeLa treated with 100ng/mL IFN gamma for 72 hours cell lysate, human tonsil tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue.
Conjugation
unconjugated
Clone Number
PSH16-55
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000-1:20,000
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IHC-P
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1:200-1:1,000
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IP
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1-2μg/sample
Target
Function
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules.
Background References
1. Grossmann N, Vakkasoglu AS, Hulpke S, Abele R, Gaudet R, Tampé R. Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter. Nat Commun. 2014 Nov 7;5:5419. doi: 10.1038/ncomms6419. PMID: 25377891; PMCID: PMC4242082.
2. Gorbulev S, Abele R, Tampé R. Allosteric crosstalk between peptide-binding, transport, and ATP hydrolysis of the ABC transporter TAP. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3732-7. doi: 10.1073/pnas.061467898. PMID: 11274390; PMCID: PMC31121.
Subcellular Location
Endoplasmic reticulum membrane.
Synonyms
ABC transporter, MHC 2 antibody
ABC18 antibody
ABCB3 antibody
Antigen peptide transporter 2 antibody
APT2 antibody
ATP binding cassette, sub family B (MDR/TAP), member 3 antibody
ATP-binding cassette sub-family B member 3 antibody
D6S217E antibody
Peptide supply factor 2 antibody
Peptide transporter involved in antigen processing 2 antibody
ExpandImages
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Western blot analysis of TAP2 on different lysates with Rabbit anti-TAP2 antibody (HA751604) at 1/20,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: MoLT-4 cell lysate (20 µg/Lane)
Lane 5: SW480 cell lysate (20 µg/Lane)
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751604) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of TAP2 on different lysates with Rabbit anti-TAP2 antibody (HA751604) at 1/20,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL IFN gamma for 72 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751604) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-TAP2 antibody (HA751604) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751604) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TAP2 antibody (HA751604) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751604) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TAP2 antibody (HA751604) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751604) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TAP2 antibody (HA751604) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751604) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
TAP2 was immunoprecipitated from 0.2 mg MOLT-4 cell lysate with HA751604 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751604 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MOLT-4 cell lysate (input)
Lane 2: HA751604 IP in MOLT-4 cell lysate
Lane 3: Rabbit IgG instead of HA751604 in MOLT-4 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"