Bcl2-L-13 Recombinant Rabbit Monoclonal Antibody [PSH17-12] - BSA and Azide free
Catalog# HA751616
Bcl2-L-13 Recombinant Rabbit Monoclonal Antibody [PSH17-12] - BSA and Azide free
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
Bcl2-L-13 Recombinant Rabbit Monoclonal Antibody [PSH17-12] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Bcl2-L-13 aa 1-350.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 53 kDa
Positive Control
K-562 cell lysate, THP-1 cell lysate, Raji cell lysate, COS-1 cell lysate, human heart tissue, human placenta tissue, mouse heart tissue, mouse liver tissue, mouse placenta tissue, rat heart tissue, rat liver tissue, rat placenta tissue.
Conjugation
unconjugated
Clone Number
PSH17-12
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
BCL2-like 13 (apoptosis facilitator), also known as BCL2L13 or Bcl-rambo, is a protein which in humans is encoded by the BCL2L13 gene on chromosome 22. This gene encodes a mitochondrially-localized protein which is classified under the Bcl-2 protein family. Overexpression of the encoded protein results in apoptosis. As a result, it has been implicated in cancers such as childhood acute lymphoblastic leukemia (ALL) and glioblastoma multiforme (GBM). Alternatively spliced transcript variants have been observed for this gene, such as Bcl-rambo beta.
Background References
1. Murakawa T et al. AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection. Cell Rep. 2024 Dec
2. Wang J et al. BCL2L13 promotes mitophagy through DNM1L-mediated mitochondrial fission in glioblastoma. Cell Death Dis. 2023 Sep
Subcellular Location
Mitochondrion membrane, Nucleus.
Synonyms
Apoptosis facilitator antibody
B2L13_HUMAN antibody
Bcl 2 like 13 antibody
Bcl 2 like 13 protein antibody
Bcl rambo antibody
Bcl-2-like protein 13 antibody
Bcl-rambo antibody
BCL2 like 13 apoptosis facilitator antibody
BCL2 like 13 antibody
Bcl2 like 13 protein antibody
ExpandImages
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This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Western blot analysis of Bcl2-L-13 on different lysates with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/50,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 85 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723899) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723899, the same antibody clone in a different buffer formulation.
Bcl2-L-13 was immunoprecipitated from 0.2 mg K-562 cell lysate with HA723899 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723899 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate (input)
Lane 2: HA723899 IP in K-562 cell lysate
Lane 3: Rabbit IgG instead of HA723899 in K-562 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"