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c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-79] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751638

c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-79] - BSA and Azide free

Application

  • IHC-Fr

  • IF-Cell

  • FC

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

c-Kit Recombinant Rabbit Monoclonal Antibody [PSH17-79] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse c-Kit aa 1-527.

Species Reactivity

Mouse

Validated Applications

IHC-Fr, IF-Cell, FC

Molecular Weight

Predicted band size: 109 kDa

Positive Control

Mouse testis tissue, ES-D3.

Conjugation

unconjugated

Clone Number

PSH17-79

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IF-Cell

  • 1:50

  • FC

  • 1:1,000

Target

Function

Proto-oncogene c-KIT is the gene encoding the receptor tyrosine kinase protein known as tyrosine-protein kinase KIT, CD117 (cluster of differentiation 117) or mast/stem cell growth factor receptor (SCFR). KIT is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. Altered forms of this receptor may be associated with some types of cancer. KIT is a receptor tyrosine kinase type III, which binds to stem cell factor, also known as "steel factor" or "c-kit ligand". When this receptor binds to stem cell factor (SCF) it forms a dimer that activates its intrinsic tyrosine kinase activity, that in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. After activation, the receptor is ubiquitinated to mark it for transport to a lysosome and eventual destruction. Signaling through KIT plays a role in cell survival, proliferation, and differentiation. For instance, KIT signaling is required for melanocyte survival, and it is also involved in haematopoiesis and gametogenesis.

Background References

1. Gao X et al. Regulation of the hematopoietic stem cell pool by C-Kit-associated trogocytosis. Science. 2024 Aug

2. Xiong Y et al. c-Kit signaling potentiates CAR T cell efficacy in solid tumors by CD28- and IL-2-independent co-stimulation. Nat Cancer. 2023 Jul

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P05532 Mouse

Synonyms

C Kit antibody

c-Kit antibody

c-Kit Ligand antibody

CD117 antibody

Kit antibody

Kit Ligand antibody

KIT oncogene antibody

KIT proto oncogene receptor tyrosine kinase antibody

Mast cell growth factor receptor antibody

Mast/stem cell growth factor receptor Kit antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: testis<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: testis

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of ES-D3 (positive) and NIH/3T3 (negative) cells labeling c-Kit with Rabbit anti-c-Kit antibody (<a href="/products/HA751638" style="font-weight: bold;text-decoration: underline;">HA751638</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Kit antibody (<a href="/products/HA751638" style="font-weight: bold;text-decoration: underline;">HA751638</a>) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Relative expression (RE)

    Immunocytochemistry analysis of ES-D3 (positive) and NIH/3T3 (negative) cells labeling c-Kit with Rabbit anti-c-Kit antibody (HA751638) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Kit antibody (HA751638) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of NIH/3T3 (left, negative) and ES-D3 (right, positive) cells labeling c-Kit.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751638" style="font-weight: bold;text-decoration: underline;">HA751638</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NIH/3T3 (left, negative) and ES-D3 (right, positive) cells labeling c-Kit.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751638, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"