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PIGR Recombinant Rabbit Monoclonal Antibody [PSH17-84] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751643

PIGR Recombinant Rabbit Monoclonal Antibody [PSH17-84] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PIGR Recombinant Rabbit Monoclonal Antibody [PSH17-84] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PIGR aa 1-661.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 83 kDa

Positive Control

A431 cell lysate, Mouse colon tissue lysate, Rat stomach tissue lysate, Rat colon tissue lysate.

Conjugation

unconjugated

Clone Number

PSH17-84

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

Polymeric immunoglobulin receptor (pIgR) is a transmembrane protein that in humans is encoded by the PIGR gene. It is an Fc receptor which facilitates the transcytosis of the soluble polymeric isoforms of immunoglobulin A and immunoglobulin M (pIg) and immune complexes. pIgRs are mainly located on the epithelial lining of mucosal surfaces of the gastrointestinal tract. The composition of the receptor is complex, including 6 immunoglobulin-like domains, a transmembrane region, and an intracellular domain. pIgR expression is under the strong regulation of cytokines, hormones, and pathogenic stimuli.

Background References

1. Chae J et al. Polymeric immunoglobulin receptor (pIgR) in cancer. J Cancer Res Clin Oncol. 2023 Dec

2. Zingoni A et al. PIGR-enriched circulating vesicles contributes to hepatocellular carcinoma aggressiveness. J Hepatol. 2022 Apr

Subcellular Location

Cell membrane; Secreted.

UNIPROT

SwissProt: P01833 Human

SwissProt: O70570 Mouse

SwissProt: P15083 Rat

Synonyms

Hepatocellular carcinoma associated protein TB6 antibody

Hepatocellular carcinoma-associated protein TB6 antibody

MGC125361 antibody

MGC125362 antibody

Phosphatidylinositol glycan, class R antibody

PIGR antibody

PIGR_HUMAN antibody

Poly Ig receptor antibody

Poly-Ig receptor antibody

Polymeric immunoglobulin receptor antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of PIGR on different lysates with Rabbit anti-PIGR antibody (<a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a>) at 1/5,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: A549 cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 83 kDa<br />Observed band size: 70-100 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of PIGR on different lysates with Rabbit anti-PIGR antibody (HA751643) at 1/5,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: A549 cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 83 kDa
    Observed band size: 70-100 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751643) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PIGR on different lysates with Rabbit anti-PIGR antibody (<a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse colon tissue lysate<br />Lane 2: Rat stomach tissue lysate<br />Lane 3: Rat colon tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 83 kDa<br />Observed band size: 70-100 kDa<br /><br />Exposure time: 42 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PIGR on different lysates with Rabbit anti-PIGR antibody (HA751643) at 1/5,000 dilution.

    Lane 1: Mouse colon tissue lysate
    Lane 2: Rat stomach tissue lysate
    Lane 3: Rat colon tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 83 kDa
    Observed band size: 70-100 kDa

    Exposure time: 42 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751643) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • PIGR was immunoprecipitated from 0.2 mg A431 cell lysate with <a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A431 cell lysate (input)<br />Lane 2: <a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a> IP in A431 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751643" style="font-weight: bold;text-decoration: underline;">HA751643</a> in A431 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    PIGR was immunoprecipitated from 0.2 mg A431 cell lysate with HA751643 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751643 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A431 cell lysate (input)
    Lane 2: HA751643 IP in A431 cell lysate
    Lane 3: Rabbit IgG instead of HA751643 in A431 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 3 minutes; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"