IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH18-00] - BSA and Azide free
Catalog# HA751649
IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH18-00] - BSA and Azide free
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WB
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IHC-P
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IF-Cell
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Mouse
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Rat
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HA723965
含抗保成分
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unconjugated
Overview
Product Name
IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH18-00] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL-1 beta aa 100-269
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 31 kDa
Positive Control
RAW264.7 treated with 100ng/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate, NR8383 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH18-00
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:1,000
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IF-Cell
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1:200-1:2,000
Target
Function
The Interleukin 1 (IL-1) family of proteins consists of IL-1 alpha, IL-1 beta, and the IL-1 receptor antagonist (IL-1ra). IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions. IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulindependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid (aa) level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa. Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide, but evidence suggests that these factors can be secreted by non-classical pathways. A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity. The precursor form of IL-1 beta, unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form. Both unprocessed and mature forms of IL-1 beta are exported from the cell.
Background References
1. Qiao J et al. Autologous platelet rich plasma injection can be effective in the management of osteoarthritis of the knee: impact on IL-1 beta, TNF-alpha, hs-CRP. J Orthop Surg Res. 2024 Oct
2. Vicens-Artés S et al. Effect of MP-AzeFlu in IL-1 beta-induced IL-6 and proinflammatory cytokines. Immunol Res. 2023 Jun
Subcellular Location
Cytoplasm, cytosol, Secreted, Lysosome, extracellular exosome.
Synonyms
Catabolin antibody
H1 antibody
IFN beta inducing factor antibody
IL 1 antibody
IL 1 beta antibody
IL-1 beta antibody
IL1 antibody
IL1 BETA antibody
IL1B antibody
IL1B_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody (HA751649) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate
Lane 3: NR8383 cell lysate
Lane 4: NR8383 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 35/20 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751649) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded RAW264.7 cells untreated / treated with 200ng/mL LPS for 4 hours add 1μg/mL BFA for 2 hours with Rabbit anti-IL-1 beta antibody (HA751649) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751649) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Immunohistochemical analysis of paraffin-embedded NR8383 cells untreated / treated with 100nM TPA overnight then treated with 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours with Rabbit anti-IL-1 beta antibody (HA751649) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751649) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 100ng/mL LPS for 4 hours add 300ng/mL BFA for 3 hours labeling IL-1 beta with Rabbit anti-IL-1 beta antibody (HA751649) at 1/5,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 beta antibody (HA751649) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of NR8383 cells untreated / treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours labeling IL-1 beta with Rabbit anti-IL-1 beta antibody (HA751649) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 beta antibody (HA751649) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"