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PADI2 Recombinant Rabbit Monoclonal Antibody [PSH18-07] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751653

PADI2 Recombinant Rabbit Monoclonal Antibody [PSH18-07] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PADI2 Recombinant Rabbit Monoclonal Antibody [PSH18-07] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PADI2 aa 1-350.

Species Reactivity

Human, Green monkey

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 76 kDa

Positive Control

PANC-1 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, HT-29 cell lysate, COS-1 cell lysate.

Conjugation

unconjugated

Clone Number

PSH18-07

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Protein-arginine deiminase type-2 is an enzyme that in humans is encoded by the PADI2 gene. This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.

Background References

1. Yu X et al. Loss of PADI2 and PADI4 ameliorates sepsis-induced acute lung injury by suppressing NLRP3+ macrophages. JCI Insight. 2024 Nov

2. Mou Z et al. Icaritin inhibits the progression of urothelial cancer by suppressing PADI2-mediated neutrophil infiltration and neutrophil extracellular trap formation. Acta Pharm Sin B. 2024 Sep

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9Y2J8 Human

Synonyms

KIAA0994 antibody

OTTHUMP00000044625 antibody

PAD 2 antibody

PAD H19 antibody

PAD-H19 antibody

PAD2 antibody

PADI 2 antibody

Padi2 antibody

PADI2 protein antibody

PADI2_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of PADI2 on different lysates with Rabbit anti-PADI2 antibody (<a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a>) at 1/5,000 dilution.<br /><br />Lane 1: PANC-1 cell lysate<br />Lane 2: SK-Br-3 cell lysate<br />Lane 3: MCF7 cell lysate (low expression)<br />Lane 4: HT-29 cell lysate<br />Lane 5: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 76 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: Lane 1-2: 6 seconds; Lane 3-5: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    This data was developed using HA723970, the same antibody clone in a different buffer formulation.
    Western blot analysis of PADI2 on different lysates with Rabbit anti-PADI2 antibody (HA723970) at 1/5,000 dilution.

    Lane 1: PANC-1 cell lysate
    Lane 2: SK-Br-3 cell lysate
    Lane 3: MCF7 cell lysate (low expression)
    Lane 4: HT-29 cell lysate
    Lane 5: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 76 kDa
    Observed band size: 75 kDa

    Exposure time: Lane 1-2: 6 seconds; Lane 3-5: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723970) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a>, the same antibody clone in a different buffer formulation.<br />PADI2 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with <a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: PANC-1 cell lysate (input)<br />Lane 2: <a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a> IP in PANC-1 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723970" style="font-weight: bold;text-decoration: underline;">HA723970</a> in PANC-1 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 2 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    This data was developed using HA723970, the same antibody clone in a different buffer formulation.
    PADI2 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA723970 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723970 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: PANC-1 cell lysate (input)
    Lane 2: HA723970 IP in PANC-1 cell lysate
    Lane 3: Rabbit IgG instead of HA723970 in PANC-1 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 2 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"