heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody [PSH18-12] - BSA and Azide free
Catalog# HA751658
heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody [PSH18-12] - BSA and Azide free
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody [PSH18-12] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within mouse MYH3 aa 1,811-1,860.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 224 kDa
Positive Control
RD cell lysate, Mouse embryo tissue lysate, Rat embryo tissue lysate, human fetal skeletal muscle tissue, mouse E14.5 embryo tissue, rat E14.5 embryo tissue.
Conjugation
unconjugated
Clone Number
PSH18-12
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Myosin-3 is a protein that in humans is encoded by the MYH3 gene. Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman–Sheldon syndrome and Sheldon–Hall syndrome.
Background References
1. Morali B et al. Bi-allelic variants in MYH3 cause recessively-inherited arthrogryposis. Clin Genet. 2024 Oct
2. Jacinto JGP et al. MYH3-associated non-syndromic palatoschisis (cleft palate, CP) in Limousine cattle. Anim Genet. 2023 Mar
Subcellular Location
Cytoplasm, myofibril.
Synonyms
embryonic antibody
fast skeletal muscle antibody
HEMHC antibody
Muscle embryonic myosin heavy chain 3 antibody
Muscle embryonic myosin heavy chain antibody
MYH 3 antibody
Myh3 antibody
MYH3_HUMAN antibody
MYHC EMB antibody
MYHSE 1 antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Western blot analysis of heavy chain Myosin / MYH3 on different lysates with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
Lane 1: RD cell lysate (20 µg/Lane)
Lane 2: Mouse embryo tissue lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (negative) (20 µg/Lane)
Lane 4: Rat embryo tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (negative) (20 µg/Lane)
Predicted band size: 224 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723975) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA723975) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723975) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA723975, the same antibody clone in a different buffer formulation.
heavy chain Myosin / MYH3 was immunoprecipitated from 0.2 mg RD cell lysate with HA723975 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723975 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RD cell lysate (input)
Lane 2: HA723975 IP in RD cell lysate
Lane 3: Rabbit IgG instead of HA723975 in RD cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 20 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"