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heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody [PSH18-12] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751658

heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody [PSH18-12] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody [PSH18-12] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within mouse MYH3 aa 1,811-1,860.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 224 kDa

Positive Control

RD cell lysate, Mouse embryo tissue lysate, Rat embryo tissue lysate, human fetal skeletal muscle tissue, mouse E14.5 embryo tissue, rat E14.5 embryo tissue.

Conjugation

unconjugated

Clone Number

PSH18-12

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:5,000-1:30,000

  • IP

  • 1-2μg/sample

Target

Function

Myosin-3 is a protein that in humans is encoded by the MYH3 gene. Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman–Sheldon syndrome and Sheldon–Hall syndrome.

Background References

1. Morali B et al. Bi-allelic variants in MYH3 cause recessively-inherited arthrogryposis. Clin Genet. 2024 Oct

2. Jacinto JGP et al. MYH3-associated non-syndromic palatoschisis (cleft palate, CP) in Limousine cattle. Anim Genet. 2023 Mar

Subcellular Location

Cytoplasm, myofibril.

UNIPROT

SwissProt: P11055 Human

SwissProt: P13541 Mouse

SwissProt: P12847 Rat

Synonyms

embryonic antibody

fast skeletal muscle antibody

HEMHC antibody

Muscle embryonic myosin heavy chain 3 antibody

Muscle embryonic myosin heavy chain antibody

MYH 3 antibody

Myh3 antibody

MYH3_HUMAN antibody

MYHC EMB antibody

MYHSE 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of heavy chain Myosin / MYH3 on different lysates with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution.<br /><br />Lane 1: RD cell lysate (20 µg/Lane)<br />Lane 2: Mouse embryo tissue lysate (20 µg/Lane)<br />Lane 3: Mouse testis tissue lysate (negative) (20 µg/Lane)<br />Lane 4: Rat embryo tissue lysate (20 µg/Lane)<br />Lane 5: Rat testis tissue lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 224 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of heavy chain Myosin / MYH3 on different lysates with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.

    Lane 1: RD cell lysate (20 µg/Lane)
    Lane 2: Mouse embryo tissue lysate (20 µg/Lane)
    Lane 3: Mouse testis tissue lysate (negative) (20 µg/Lane)
    Lane 4: Rat embryo tissue lysate (20 µg/Lane)
    Lane 5: Rat testis tissue lysate (negative) (20 µg/Lane)

    Predicted band size: 224 kDa
    Observed band size: 250 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751658) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751658) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/30,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/30,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751658) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751658) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751658) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751658) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat adult skeletal muscle tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751658) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue (negative) with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751658) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • heavy chain Myosin / MYH3 was immunoprecipitated from 0.2 mg RD cell lysate with <a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: RD cell lysate (input)<br />Lane 2: <a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a> IP in RD cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751658" style="font-weight: bold;text-decoration: underline;">HA751658</a> in RD cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 20 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    heavy chain Myosin / MYH3 was immunoprecipitated from 0.2 mg RD cell lysate with HA751658 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751658 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: RD cell lysate (input)
    Lane 2: HA751658 IP in RD cell lysate
    Lane 3: Rabbit IgG instead of HA751658 in RD cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 20 seconds; ECL: K1801

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