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Ly6g Recombinant Rabbit Monoclonal Antibody [PSH18-48] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751672

Ly6g Recombinant Rabbit Monoclonal Antibody [PSH18-48] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Ly6g Recombinant Rabbit Monoclonal Antibody [PSH18-48] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Ly6g aa 1-105.

Species Reactivity

Mouse

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 14 kDa

Positive Control

Mouse spleen tissue lysate, Mouse lung tissue lysate, mouse bone marrow tissue, mouse spleen tissue, mouse lung tissue.

Conjugation

unconjugated

Clone Number

PSH18-48

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:5,000-1:10,000

Target

Function

Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

Background References

1. Ruscitti C et al. Recruited atypical Ly6G(+) macrophages license alveolar regeneration after lung injury. Sci Immunol. 2024 Aug

2. Iliakis CS et al. Never trust a single myeloid marker: Ly6G on repair-promoting lung macrophages. Sci Immunol. 2024 Aug

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P35461 Mouse

Synonyms

Gr-1 antibody

Gr1 antibody

Ly-6G.1 antibody

Ly6G antibody

Lymphocyte antigen 6 complex locus G antibody

Lymphocyte antigen 6G antibody

Images

  • Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse lung tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (HA751672) at 1/5,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse lung tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 25 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751672) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue with Rabbit anti-Ly6g antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue with Rabbit anti-Ly6g antibody (HA751672) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751672) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ly6g antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ly6g antibody (HA751672) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751672) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ly6g antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ly6g antibody (HA751672) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751672) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T transfected with empty control cell lysate (negative)<br />Lane 2: 293T transfected with Ly6g cell lysate (positive)<br />Lane 3: 293T transfected with Ly6f cell lysate (negative)<br />Lane 4: 293T transfected with Ly6i cell lysate (negative)<br />Lane 5: 293T transfected with Ly6c1 cell lysate (negative)<br />Lane 6: 293T transfected with Ly6c2 cell lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751672" style="font-weight: bold;text-decoration: underline;">HA751672</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (HA751672) at 1/5,000 dilution.

    Lane 1: 293T transfected with empty control cell lysate (negative)
    Lane 2: 293T transfected with Ly6g cell lysate (positive)
    Lane 3: 293T transfected with Ly6f cell lysate (negative)
    Lane 4: 293T transfected with Ly6i cell lysate (negative)
    Lane 5: 293T transfected with Ly6c1 cell lysate (negative)
    Lane 6: 293T transfected with Ly6c2 cell lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751672) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"