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Cystatin C Recombinant Rabbit Monoclonal Antibody [PSH18-60] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751684

Cystatin C Recombinant Rabbit Monoclonal Antibody [PSH18-60] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Cystatin C Recombinant Rabbit Monoclonal Antibody [PSH18-60] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Cystatin C aa 1-146.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 16 kDa

Positive Control

MDA-MB-231 cell lysate, Huh7 cell lysate, TT cell lysate, human kidney tissue, human ovary tissue, mouse kidney tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH18-60

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy.

Background References

1. Chen DC et al. Advantages, Limitations, and Clinical Considerations in Using Cystatin C to Estimate GFR. Kidney360. 2022 Aug

2. Skidmore M et al. Cystatin C as a Marker of Kidney Function in Children. Biomolecules. 2024 Aug

Subcellular Location

Secreted

UNIPROT

SwissProt:P01034 Human

SwissProt:P21460 Mouse

SwissProt:P14841 Rat

Synonyms

Cystatin-C

Cystatin-3

Gamma-trace

Neuroendocrine basic polypeptide

Post-gamma-globulin

CST3

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/20,000 dilution.<br /><br />Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 2: MOLT-4 cell lysate (negative) (20 µg/Lane)<br />Lane 3: Huh7 cell lysate (20 µg/Lane)<br />Lane 4: TT cell lysate (20 µg/Lane)<br /><br />Predicted band size: 16 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/20,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    This data was developed using HA724013, the same antibody clone in a different buffer formulation.
    Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA724013) at 1/20,000 dilution.

    Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 2: MOLT-4 cell lysate (negative) (20 µg/Lane)
    Lane 3: Huh7 cell lysate (20 µg/Lane)
    Lane 4: TT cell lysate (20 µg/Lane)

    Predicted band size: 16 kDa
    Observed band size: 14 kDa

    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724013) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cystatin C antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA724013, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Cystatin C antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA724013, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cystatin C antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA724013, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/20,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (10 µg/Lane)<br />Lane 2: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16/18 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724013" style="font-weight: bold;text-decoration: underline;">HA724013</a>) at 1/20,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA724013, the same antibody clone in a different buffer formulation.
    Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA724013) at 1/20,000 dilution.

    Lane 1: Mouse brain tissue lysate (10 µg/Lane)
    Lane 2: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 16 kDa
    Observed band size: 16/18 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724013) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"