MuERVL-Gag Recombinant Rabbit Monoclonal Antibody [PSH19-35] - BSA and Azide free
Catalog# HA751705
MuERVL-Gag Recombinant Rabbit Monoclonal Antibody [PSH19-35] - BSA and Azide free
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WB
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IF-Cell
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IP
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Mouse
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unconjugated
Overview
Product Name
MuERVL-Gag Recombinant Rabbit Monoclonal Antibody [PSH19-35] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse Gag aa 201-581.
Species Reactivity
Mouse
Validated Applications
WB, IF-Cell, IP
Target Molecular Weight
Predicted band size: 67 kDa
Positive Control
ES-D3 cell lysate, ES-W4 cell lysate.
Conjugation
unconjugated
Clone Number
PSH19-35
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100
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IP
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1-2μg/sample
Target
Function
A large number of retrotransposons are expressed when the zygotic genome is first transcribed, including the endogenous retroviruses (ERVs), LINE-1 elements, and the non-autonomous SINE elements. At the 2C stage, MuERV-L/MERVL retrovirus-like elements are transiently de-repressed and produce 3% of the transcribed mRNAs. Following the 2C stage, MERVL-retroelement expression is silenced. The newly study discovered that this regulated pattern of MERVL expression overlapped with greater than one hundred 2C-specific genes that have co-opted regulatory elements from these foreign retroviruses to initiate their transcription.
Background References
暂无
UNIPROT
Images
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Western blot analysis of MuERVL-Gag on different lysates with Rabbit anti-MuERVL-Gag antibody (HA751705) at 1/5,000 dilution.
Lane 1: ES-D3 cell lysate
Lane 2: ES-W4 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751705) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
MuERVL-Gag was immunoprecipitated from 0.2 mg ES-W4 cell lysate with HA751705 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751705 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: ES-W4 cell lysate (input)
Lane 2: HA751705 IP in ES-W4 cell lysate
Lane 3: Rabbit IgG instead of HA751705 in ES-W4 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 2 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"