CD130 / GP130 Recombinant Rabbit Monoclonal Antibody [PSH20-62] - BSA and Azide free
Catalog# HA751763
CD130 / GP130 Recombinant Rabbit Monoclonal Antibody [PSH20-62] - BSA and Azide free
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IHC-Fr
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FC
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Mouse
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unconjugated
Overview
Product Name
CD130 / GP130 Recombinant Rabbit Monoclonal Antibody [PSH20-62] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse CD130 / GP130 aa 1-617.
Species Reactivity
Mouse
Validated Applications
IHC-Fr, FC
Target Molecular Weight
Predicted band size: 102 kDa
Positive Control
Mouse spleen, C57BL/6 mouse splenocytes.
Conjugation
unconjugated
Clone Number
PSH20-62
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:500
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FC
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1:1,000
Target
Function
Glycoprotein 130 (also known as gp130, IL6ST, IL6R-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of the type I cytokine receptor within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement. As with other type I cytokine receptors, gp130 possesses a WSXWS amino acid motif that ensures correct protein folding and ligand binding. It interacts with Janus kinases to elicit an intracellular signal following receptor interaction with its ligand. Structurally, gp130 is composed of five fibronectin type-III domains and one immunoglobulin-like C2-type (immunoglobulin-like) domain in its extracellular portion.
Background References
1. Zanders L et al. Sepsis induces interleukin 6, gp130/JAK2/STAT3, and muscle wasting. J Cachexia Sarcopenia Muscle. 2022 Feb
2. Zhu X et al. Intermittent Fasting-Induced Orm2 Promotes Adipose Browning via the GP130/IL23R-p38 Cascade. Adv Sci (Weinh). 2024 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD130 antibody
CD130 antigen antibody
CDw130 antibody
gp130 antibody
GP130 RAPS antibody
IL 6R beta antibody
IL-6 receptor subunit beta antibody
IL-6R subunit beta antibody
IL-6R-beta antibody
IL-6RB antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: spleen
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Flow cytometric analysis of C57BL/6 mouse splenocytes labeling CD130 / GP130 (HA751763) and CD3 (HA600118F, APC).
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751763, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Rabbit IgG Isotype Control (iFluor™ 488, 1/1,000).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"