CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93] - BSA and Azide free
Catalog# HA751775
CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93] - BSA and Azide free
-
IF-Cell
-
FC
-
Mouse
-
unconjugated
Overview
Product Name
CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse CD38 aa 1-304.
Species Reactivity
Mouse
Validated Applications
IF-Cell, FC
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
Mouse splenocytes.
Conjugation
unconjugated
Clone Number
PSH20-93
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IF-Cell
-
1:500
-
FC
-
1:1,000
Target
Function
CD38 is a type II integral membrane glycoprotein which is present on early B and T cell lineages and activated B and T cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, monocytes and Ig-secreting plasma cells. CD38 acts as a NAD glycohydrolase in T lymphocytes. On hematopoietic cells CD38 induces activation, proliferation, and differentiation of mature T and B cells and mediates apoptosis of myeloid and lymphoid progenitor cells. In addition to acting as a signaling receptor, CD38 is also an enzyme capable of producing several calcium-mobilizing metabolites, including cyclic adenosine diphosphate ribose (cADPR). CD38 also plays a role in maintaining survival of an invariant NK T (iNKT) cell subset that preferentially contributes to the maintenance of immunological tolerance.
Background References
1. Yang Q et al. NADase CD38 is a key determinant of ovarian aging. Nat Aging. 2024 Jan
2. Yu S et al. CD38-Targeting Peptide Vaccine Ameliorates Aging-Associated Phenotypes in Mice. Aging Cell. 2025 Sep
Subcellular Location
Membrane.
UNIPROT
Synonyms
Acute lymphoblastic leukemia cells antigen CD38 antibody
ADP ribosyl cyclase 1 antibody
ADP ribosyl cyclase antibody
ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
ADP-ribosyl cyclase 1 antibody
ADPRC 1 antibody
ADPRC1 antibody
cADPr hydrolase 1 antibody
CD 38 antibody
CD38 antibody
ExpandImages
-
Immunocytochemistry analysis of mouse splenocytes labeling CD38 with Rabbit anti-CD38 antibody (HA751775) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (HA751775) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of mouse splenocytes labeling CD38.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751775, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"