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NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751840

NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10] - BSA and Azide free

Application

  • WB

  • IP

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human NeuroD1 aa 1-44 / 356.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IP, IHC-P, IHC-Fr

Target Molecular Weight

Predicted band size: 40 kDa

Positive Control

Mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue, Mouse cerebellum (P7) tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.

Conjugation

unconjugated

Clone Number

JM11-10

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. The HLH domain is required for dimerization, while the basic region makes specific contacts with DNA. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains. These proteins heterodimerize with members of the E protein family and initiate myogenesis. Neuro D has been identified as a bHLH transcription factor functioning in neurogenic differentiation. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.

Background References

1. Zhang F et al. Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells. Neurosci Bull 32:205-16 (2016).

2. Borromeo MD et al. ASCL1 and NEUROD1 Reveal Heterogeneity in Pulmonary Neuroendocrine Tumors and Regulate Distinct Genetic Programs. Cell Rep 16:1259-72 (2016).

Post-translational Modification

Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells (By similarity).

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: Q13562 Human

SwissProt: Q60867 Mouse

SwissProt: Q64289 Rat

Synonyms

atonal antibody

basic helix loop helix transcription factor antibody

BETA 2 antibody

Beta cell E box transactivator 2 antibody

BETA2 antibody

BHF 1 antibody

BHF1 antibody

bHLHa3 antibody

class A basic helix loop helix protein 3 antibody

Class A basic helix-loop-helix protein 3 antibody

Expand

Images

  • This data was developed using <a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:100<br /><br />Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    This data was developed using ET1703-73, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:100

    Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • This data was developed using <a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:100<br /><br />Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    This data was developed using ET1703-73, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:100

    Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • This data was developed using <a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of NeuroD1 on different lysates with Rabbit anti-NeuroD1 antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse cerebellum (P7) tissue lysate (40 µg/Lane)<br />Lane 2: Mouse cerebellum tissue lysate (no heat) (40 µg/Lane)<br />Lane 3: Mouse liver tissue lysate (negative) (40 µg/Lane)<br />Lane 4: Rat cerebellum tissue lysate (40 µg/Lane)<br />Lane 5: Rat liver tissue lysate (negative) (40 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br />Predicted band size: 40 kDa<br />Observed band size: 50 kDa<br />Exposure time: 51 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using ET1703-73, the same antibody clone in a different buffer formulation.
    Western blot analysis of NeuroD1 on different lysates with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/1,000 dilution.

    Lane 1: Mouse cerebellum (P7) tissue lysate (40 µg/Lane)
    Lane 2: Mouse cerebellum tissue lysate (no heat) (40 µg/Lane)
    Lane 3: Mouse liver tissue lysate (negative) (40 µg/Lane)
    Lane 4: Rat cerebellum tissue lysate (40 µg/Lane)
    Lane 5: Rat liver tissue lysate (negative) (40 µg/Lane)

    Notice: no heat means the lysate is not boiled.
    Predicted band size: 40 kDa
    Observed band size: 50 kDa
    Exposure time: 51 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-73) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NeuroD1 antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1703-73, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NeuroD1 antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1703-73, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NeuroD1 antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-73" style="font-weight: bold;text-decoration: underline;">ET1703-73</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1703-73, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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