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Laminin beta 1 Recombinant Rabbit Monoclonal Antibody [JM099-03] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751844

Laminin beta 1 Recombinant Rabbit Monoclonal Antibody [JM099-03] - BSA and Azide free

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Laminin beta 1 Recombinant Rabbit Monoclonal Antibody [JM099-03] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Laminin beta 1 aa 1,721-1,769 / 1,786.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 198 kDa

Positive Control

SW480 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, Mouse stomach tissue lysate, Rat stomach tissue lysate, HepG2 cell lysate, Rat heart tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JM099-03

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:1,000

Target

Function

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms, which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1.

Background References

1. Yuan X et al. Histone acetylation is involved in TCDD-induced cleft palate formation in fetal mice. Mol Med Rep 14:1139-45 (2016).

2. Little GH et al. Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Nucleic Acids Res 40:3538-47 (2012).

Subcellular Location

Secreted.

UNIPROT

SwissProt: P07942 Human

SwissProt: P02469 Mouse

Entrez Gene: 298941 Rat

Synonyms

CLM antibody

Cutis laxa with marfanoid phenotype antibody

LAM B1 antibody

LAMB 1 antibody

LAMB1 antibody

LAMB1_HUMAN antibody

Laminin B1 antibody

Laminin B1 chain antibody

Laminin beta 1 chain antibody

Laminin beta 1 chain precursor antibody

Expand

Images

  • Western blot analysis of Laminin beta 1 on different lysates with Rabbit anti-Laminin beta 1 antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/5,000 dilution.<br /><br />Lane 1: SW480 cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: MEF cell lysate<br />Lane 4: C6 cell lysate<br />Lane 5: Mouse stomach tissue lysate<br />Lane 6: Rat stomach tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 198 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Laminin beta 1 on different lysates with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/5,000 dilution.

    Lane 1: SW480 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: MEF cell lysate
    Lane 4: C6 cell lysate
    Lane 5: Mouse stomach tissue lysate
    Lane 6: Rat stomach tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 198 kDa
    Observed band size: 250 kDa

    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751844) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Laminin beta 1 on different lysates with Rabbit anti-Laminin beta 1 antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (15 µg/Lane)<br />Lane 2: C6 cell lysate (15 µg/Lane)<br />Lane 3: Mouse stomach tissue lysate (20 µg/Lane)<br />Lane 4: Rat heart tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 198 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 3 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Laminin beta 1 on different lysates with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate (15 µg/Lane)
    Lane 2: C6 cell lysate (15 µg/Lane)
    Lane 3: Mouse stomach tissue lysate (20 µg/Lane)
    Lane 4: Rat heart tissue lysate (20 µg/Lane)

    Predicted band size: 198 kDa
    Observed band size: 250 kDa

    Exposure time: 3 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751844) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Laminin beta 1 antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751844) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Laminin beta 1 antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751844) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Laminin beta 1 antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751844" style="font-weight: bold;text-decoration: underline;">HA751844</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Laminin beta 1 antibody (HA751844) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751844) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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